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New page: left|200px<br /><applet load="1xt6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xt6, resolution 1.80Å" /> '''S35C Flavodoxin Muta...
 
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[[Image:1xt6.gif|left|200px]]<br /><applet load="1xt6" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1xt6, resolution 1.80&Aring;" />
'''S35C Flavodoxin Mutant in the semiquinone state'''<br />


==Overview==
==S35C Flavodoxin Mutant in the semiquinone state==
The crystallographic structure of an engineered flavodoxin mutant from, Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was, used to substitute serine 35 with a cysteine to provide a possible, covalent linkage. The crystal structure of the semiquinone form of this, mutant is similar to the corresponding oxidation state of the wild-type, flavodoxin. Analysis of the structural changes reveals the interaction, between N(5)H of the flavin and the carbonyl O atom of Gly61 to be, critical for modulation of the electrochemical properties of the protein.
<StructureSection load='1xt6' size='340' side='right'caption='[[1xt6]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1xt6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XT6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xt6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xt6 OCA], [https://pdbe.org/1xt6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xt6 RCSB], [https://www.ebi.ac.uk/pdbsum/1xt6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xt6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FLAV_DESVH FLAV_DESVH] Low-potential electron donor to a number of redox enzymes.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xt/1xt6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xt6 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1XT6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with FMN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XT6 OCA].
*[[Flavodoxin 3D structures|Flavodoxin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of S35C flavodoxin mutant from Desulfovibrio vulgaris in the semiquinone state., Artali R, Marchini N, Meneghetti F, Cavazzini D, Cassetta A, Sassone C, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):481-4. Epub 2005, Mar 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15805604 15805604]
[[Category: Desulfovibrio vulgaris str. Hildenborough]]
[[Category: Desulfovibrio vulgaris]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Artali R]]
[[Category: Artali, R.]]
[[Category: Bombieri G]]
[[Category: Bombieri, G.]]
[[Category: Cassetta A]]
[[Category: Cassetta, A.]]
[[Category: Cavazzini D]]
[[Category: Cavazzini, D.]]
[[Category: Gilardi G]]
[[Category: Gilardi, G.]]
[[Category: Marchini N]]
[[Category: Marchini, N.]]
[[Category: Meneghetti F]]
[[Category: Meneghetti, F.]]
[[Category: Rossi GL]]
[[Category: Rossi, G.L.]]
[[Category: Sassone C]]
[[Category: Sassone, C.]]
[[Category: FMN]]
[[Category: flavodoxin]]
[[Category: mutant]]
[[Category: protein]]
[[Category: s35c]]
 
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Retrieved from "https://proteopedia.org/1xt6"