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New page: left|200px<br /><applet load="1y29" size="450" color="white" frame="true" align="right" spinBox="true" caption="1y29" /> '''Three dimensional solution structure of huwe...
 
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[[Image:1y29.gif|left|200px]]<br /><applet load="1y29" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1y29" />
'''Three dimensional solution structure of huwentoxin-x by 2D 1H-NMR'''<br />


==Overview==
==Three dimensional solution structure of huwentoxin-x by 2D 1H-NMR==
Huwentoxin-X (HWTX-X) is a novel peptide toxin, purified from the venom of, the spider Ornithoctonus huwena. It comprises 28 amino acid residues, including six cysteine residues as disulfide bridges linked in the pattern, of I-IV, II-V, and III-VI. Its cDNA, determined by rapid amplification of, 3' and 5' cDNA ends, encodes a 65-residue prepropeptide. HWTX-X shares low, sequence homology with omega-conotoxins GVIA and MVIIA, two well known, blockers of N-type Ca2+ channels. Nevertheless, whole cell studies, indicate that it can block N-type Ca2+ channels in rat dorsal root, ganglion cells (IC50 40 nm) and the blockage by HWTX-X is completely, reversible. The rank order of specificity for N-type Ca2+ channels is GVIA, approximately HWTX-X &gt; MVIIA. In contrast to GVIA and MVIIA, HWTX-X had no, detectable effect on the twitch response of rat vas deferens to low, frequency electrical stimulation, indicating that HWTX-X has different, selectivity for isoforms of N-type Ca2+ channels, compared with GVIA or, MVIIA. A comparison of the structures of HWTX-X and GVIA reveals that they, not only adopt a common structural motif (inhibitor cystine knot), but, also have a similar functional motif, a binding surface formed by the, critical residue Tyr, and several basic residues. However, the, dissimilarities of their binding surfaces provide some insights into their, different selectivities for isoforms of N-type Ca2+ channels.
<StructureSection load='1y29' size='340' side='right'caption='[[1y29]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1y29]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyriopagopus_schmidti Cyriopagopus schmidti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y29 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y29 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y29 OCA], [https://pdbe.org/1y29 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y29 RCSB], [https://www.ebi.ac.uk/pdbsum/1y29 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y29 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TXH10_CYRSC TXH10_CYRSC] Reversibly blocks N-type calcium channels (Cav2.2/CACNA1B) in rat dorsal root ganglion cells (IC(50)=40 nM). Elicits no toxic symptoms in either vertebrates or invertebrates during a period of 48 hours post-injection, when it was assayed in vivo by direct injection into mice and cockroaches.<ref>PMID:16439354</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Huwentoxin-X (HWTX-X) is a novel peptide toxin, purified from the venom of the spider Ornithoctonus huwena. It comprises 28 amino acid residues including six cysteine residues as disulfide bridges linked in the pattern of I-IV, II-V, and III-VI. Its cDNA, determined by rapid amplification of 3' and 5' cDNA ends, encodes a 65-residue prepropeptide. HWTX-X shares low sequence homology with omega-conotoxins GVIA and MVIIA, two well known blockers of N-type Ca2+ channels. Nevertheless, whole cell studies indicate that it can block N-type Ca2+ channels in rat dorsal root ganglion cells (IC50 40 nm) and the blockage by HWTX-X is completely reversible. The rank order of specificity for N-type Ca2+ channels is GVIA approximately HWTX-X &gt; MVIIA. In contrast to GVIA and MVIIA, HWTX-X had no detectable effect on the twitch response of rat vas deferens to low frequency electrical stimulation, indicating that HWTX-X has different selectivity for isoforms of N-type Ca2+ channels, compared with GVIA or MVIIA. A comparison of the structures of HWTX-X and GVIA reveals that they not only adopt a common structural motif (inhibitor cystine knot), but also have a similar functional motif, a binding surface formed by the critical residue Tyr, and several basic residues. However, the dissimilarities of their binding surfaces provide some insights into their different selectivities for isoforms of N-type Ca2+ channels.


==About this Structure==
Function and solution structure of Huwentoxin-X, a specific blocker of N-type calcium channels, from the Chinese bird spider Ornithoctonus huwena.,Liu Z, Dai J, Dai L, Deng M, Hu Z, Hu W, Liang S J Biol Chem. 2006 Mar 31;281(13):8628-35. Epub 2006 Jan 26. PMID:16439354<ref>PMID:16439354</ref>
1Y29 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Y29 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Function and solution structure of Huwentoxin-X, a specific blocker of N-type calcium channels, from the Chinese bird spider Ornithoctonus huwena., Liu Z, Dai J, Dai L, Deng M, Hu Z, Hu W, Liang S, J Biol Chem. 2006 Mar 31;281(13):8628-35. Epub 2006 Jan 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16439354 16439354]
</div>
[[Category: Single protein]]
<div class="pdbe-citations 1y29" style="background-color:#fffaf0;"></div>
[[Category: Liang, S.]]
== References ==
[[Category: Liu, Z.]]
<references/>
[[Category: a double-stranded beta-sheet]]
__TOC__
[[Category: ick]]
</StructureSection>
[[Category: knottin]]
[[Category: Cyriopagopus schmidti]]
 
[[Category: Large Structures]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:30:53 2007''
[[Category: Liang S]]
[[Category: Liu Z]]
Retrieved from "https://proteopedia.org/1y29"