1y4o: Difference between revisions
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New page: left|200px<br /><applet load="1y4o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1y4o" /> '''Solution structure of a mouse cytoplasmic Ro... |
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== | ==Solution structure of a mouse cytoplasmic Roadblock/LC7 dynein light chain== | ||
Roadblock/LC7 is a member of a class of dynein light chains involved in | <StructureSection load='1y4o' size='340' side='right'caption='[[1y4o]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1y4o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y4O FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y4o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4o OCA], [https://pdbe.org/1y4o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y4o RCSB], [https://www.ebi.ac.uk/pdbsum/1y4o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y4o ProSAT], [https://www.topsan.org/Proteins/CESG/1y4o TOPSAN]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/DLRB1_MOUSE DLRB1_MOUSE] Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y4/1y4o_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y4o ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Roadblock/LC7 is a member of a class of dynein light chains involved in regulating the function of the dynein complex. We have determined the three-dimensional structure of isoform 1 of the mouse Roadblock/LC7 cytoplasmic dynein light chain (robl1_mouse) by NMR spectroscopy. In contrast to a previously reported NMR structure of the human homolog with 96% sequence identity (PDB 1TGQ), which showed the protein as a monomer, our results indicate clearly that robl1 exists as a symmetric homodimer. The two beta3-strands pair with each other and form a continuous ten-stranded beta-sheet. The 25-residue alpha2-helix from one subunit packs antiparallel to that of the other subunit on the face of the beta-sheet. Zipper-like hydrophobic contacts between the two helices serve to stabilize the dimer. Through an NMR titration experiment, we localized the site on robl1_mouse that interacts with the 40 residue peptide spanning residues 243 through 282 of IC74-1_rat. These results provide physical evidence for a symmetrical interaction between dimeric robl1 and the two molecules of IC74-1 in the dynein complex. | |||
Solution structure of isoform 1 of Roadblock/LC7, a light chain in the dynein complex.,Song J, Tyler RC, Lee MS, Tyler EM, Markley JL J Mol Biol. 2005 Dec 16;354(5):1043-51. Epub 2005 Nov 2. PMID:16289575<ref>PMID:16289575</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1y4o" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Dynein 3D structures|Dynein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Lee MS]] | |||
[[Category: Markley JL]] | |||
[[Category: Lee | [[Category: Song J]] | ||
[[Category: Markley | [[Category: Tyler EM]] | ||
[[Category: Song | [[Category: Tyler RC]] | ||
[[Category: Tyler | |||
[[Category: Tyler | |||
Latest revision as of 08:00, 15 May 2024
Solution structure of a mouse cytoplasmic Roadblock/LC7 dynein light chain
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