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| [[Image:1yh1.gif|left|200px]]<br /><applet load="1yh1" size="450" color="white" frame="true" align="right" spinBox="true" | | #REDIRECT [[3kzk]] This PDB entry is obsolete and replaced by 3kzk |
| caption="1yh1, resolution 1.90Å" />
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| '''Crystal Structure of Acetylornithine Transcarbamylase'''<br />
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| ==Overview==
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| We have identified in Xanthomonas campestris a novel N-acetylornithine, transcarbamylase that replaces ornithine transcarbamylase in the canonic, arginine biosynthetic pathway of several Eubacteria. The crystal, structures of the protein in the presence and absence of the reaction, product, N-acetylcitrulline, were determined. This new family of, transcarbamylases lacks the DxxSMG motif that is characteristic of all, ornithine transcarbamylases (OTCases) and contains a novel proline-rich, loop that forms part of the active site. The specificity for, N-acetylornithine is conferred by hydrogen bonding with residues in the, proline-rich loop via water molecules and by hydrophobic interactions with, residues from the adjacent 80's, 120's, and proline-rich loops. This novel, protein structure provides a starting point for rational design of, specific analogs that may be useful in combating human and plant pathogens, that utilize acetylornithine transcarbamylase rather than ornithine, transcarbamylase.
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| ==About this Structure==
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| 1YH1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris] with SO4 and OLN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YH1 OCA].
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| ==Reference==
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| Crystal structure of N-acetylornithine transcarbamylase from Xanthomonas campestris: a novel enzyme in a new arginine biosynthetic pathway found in several eubacteria., Shi D, Morizono H, Yu X, Roth L, Caldovic L, Allewell NM, Malamy MH, Tuchman M, J Biol Chem. 2005 Apr 15;280(15):14366-9. Epub 2005 Feb 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15731101 15731101]
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| [[Category: Single protein]]
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| [[Category: Xanthomonas campestris]]
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| [[Category: Allewell, N.M.]]
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| [[Category: Caldovic, L.]]
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| [[Category: Malamy, M.H.]]
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| [[Category: Morizono, H.]]
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| [[Category: Roth, L.]]
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| [[Category: Shi, D.]]
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| [[Category: Tuchman, M.]]
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| [[Category: Yu, X.]]
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| [[Category: OLN]]
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| [[Category: SO4]]
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| [[Category: acetylcitrulline]]
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| [[Category: acetylornithine]]
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| [[Category: argf]]
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| [[Category: transcarbamylase]]
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| ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 06:46:09 2007''
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This PDB entry is obsolete and replaced by 3kzk
