2zsv: Difference between revisions

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{{Seed}}
[[Image:2zsv.png|left|200px]]


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==Crystal structure of H-2Kb in complex with JHMV epitope S598==
The line below this paragraph, containing "STRUCTURE_2zsv", creates the "Structure Box" on the page.
<StructureSection load='2zsv' size='340' side='right'caption='[[2zsv]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2zsv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Murine_hepatitis_virus_strain_JHM Murine hepatitis virus strain JHM] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZSV FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABA:ALPHA-AMINOBUTYRIC+ACID'>ABA</scene>, <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
{{STRUCTURE_2zsv|  PDB=2zsv  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zsv OCA], [https://pdbe.org/2zsv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zsv RCSB], [https://www.ebi.ac.uk/pdbsum/2zsv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zsv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HA1B_MOUSE HA1B_MOUSE] Involved in the presentation of foreign antigens to the immune system.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zs/2zsv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zsv ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
High affinity antigen-specific T cells play a critical role during protective immune responses. Epitope enhancement can elicit more potent T cell responses and can subsequently lead to a stronger memory pool; however, the molecular basis of such enhancement is unclear. We used the consensus peptide-binding motif for the Major Histocompatibility Complex molecule H-2K(b) to design a heteroclitic version of the mouse hepatitis virus-specific subdominant S598 determinant. We demonstrate that a single amino acid substitution at a secondary anchor residue (Q to Y at position 3) increased the stability of the engineered determinant in complex with H-2K(b). The structural basis for this enhanced stability was associated with local alterations in the pMHC conformation as a result of the Q to Y substitution. Recombinant viruses encoding this engineered determinant primed CTL responses that also reacted to the wildtype epitope with significantly higher functional avidity, and protected against selection of virus mutated at a second CTL determinant and consequent disease progression in persistently infected mice. Collectively, our findings provide a basis for the enhanced immunogenicity of an engineered determinant that will serve as a template for guiding the development of heteroclitic T cell determinants with applications in prevention of CTL escape in chronic viral infections as well as in tumor immunity.


===Crystal structure of H-2Kb in complex with JHMV epitope S598===
Prevention of cytotoxic T cell escape using a heteroclitic subdominant viral T cell determinant.,Butler NS, Theodossis A, Webb AI, Nastovska R, Ramarathinam SH, Dunstone MA, Rossjohn J, Purcell AW, Perlman S PLoS Pathog. 2008 Oct;4(10):e1000186. Epub 2008 Oct 24. PMID:18949029<ref>PMID:18949029</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2zsv" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_18949029}}, adds the Publication Abstract to the page
*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 18949029 is the PubMed ID number.
*[[MHC 3D structures|MHC 3D structures]]
-->
*[[MHC I 3D structures|MHC I 3D structures]]
{{ABSTRACT_PUBMED_18949029}}
== References ==
 
<references/>
==About this Structure==
__TOC__
2ZSV is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZSV OCA].
</StructureSection>
 
[[Category: Large Structures]]
==Reference==
[[Category: Murine hepatitis virus strain JHM]]
<ref group="xtra">PMID:18949029</ref><references group="xtra"/>
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Dunstone, M A.]]
[[Category: Dunstone MA]]
[[Category: Rossjohn, J.]]
[[Category: Rossjohn J]]
[[Category: Theodossis, A.]]
[[Category: Theodossis A]]
[[Category: Glycoprotein]]
[[Category: Ig fold]]
[[Category: Immune response]]
[[Category: Immune system]]
[[Category: Immunoglobulin domain]]
[[Category: Membrane]]
[[Category: Mhc i]]
[[Category: Polymorphism]]
[[Category: Protein-protein interaction]]
[[Category: Secreted]]
[[Category: Subdominant epitope]]
[[Category: Transmembrane]]
 
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Retrieved from "https://proteopedia.org/2zsv"