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New page: left|200px<br /><applet load="1yy3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yy3, resolution 2.88Å" /> '''Structure of S-Adeno...
 
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[[Image:1yy3.gif|left|200px]]<br /><applet load="1yy3" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1yy3, resolution 2.88&Aring;" />
'''Structure of S-Adenosylmethionine:tRNA Ribosyltransferase-Isomerase (QueA)'''<br />


==Overview==
==Structure of S-Adenosylmethionine:tRNA Ribosyltransferase-Isomerase (QueA)==
The enzyme S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA), is involved in the biosynthesis of the hypermodified tRNA nucleoside, queuosine. It is unprecedented in nature as it uses the cofactor, S-adenosylmethionine as the donor of a ribosyl group. We have determined, the crystal structure of Bacillus subtilis QueA at a resolution of 2.9A., The structure reveals two domains representing a 6-stranded beta-barrel, and an alpha beta alpha-sandwich, respectively. All amino acid residues, invariant in the QueA enzymes of known sequence cluster at the interface, of the two domains indicating the localization of the substrate binding, region and active center. Comparison of the B. subtilis QueA structure, with the structure of QueA from Thermotoga maritima suggests a high domain, flexibility of this enzyme.
<StructureSection load='1yy3' size='340' side='right'caption='[[1yy3]], [[Resolution|resolution]] 2.88&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1yy3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YY3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YY3 FirstGlance]. <br>
1YY3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YY3 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.88&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yy3 OCA], [https://pdbe.org/1yy3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yy3 RCSB], [https://www.ebi.ac.uk/pdbsum/1yy3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yy3 ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of Bacillus subtilis S-adenosylmethionine:tRNA ribosyltransferase-isomerase., Grimm C, Ficner R, Sgraja T, Haebel P, Klebe G, Reuter K, Biochem Biophys Res Commun. 2006 Dec 22;351(3):695-701. Epub 2006 Oct 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17083917 17083917]
== Function ==
[https://www.uniprot.org/uniprot/QUEA_BACSU QUEA_BACSU] Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yy/1yy3_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yy3 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Ficner, R.]]
[[Category: Ficner R]]
[[Category: Grimm, C.]]
[[Category: Grimm C]]
[[Category: Reuter, K.]]
[[Category: Reuter K]]
[[Category: bacillus subtilis]]
[[Category: beta-barrel]]
[[Category: quea]]
[[Category: quein queuosine]]
[[Category: s-adenosylmethionine:trna ribosyltransferase-isomerase]]
[[Category: trna-modification]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:07:39 2007''

Latest revision as of 13:38, 13 March 2024

Structure of S-Adenosylmethionine:tRNA Ribosyltransferase-Isomerase (QueA)

1yy3, resolution 2.88Å

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