2gnu: Difference between revisions

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{{Seed}}
[[Image:2gnu.png|left|200px]]


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==The crystallization of reaction center from Rhodobacter sphaeroides occurs via a new route==
The line below this paragraph, containing "STRUCTURE_2gnu", creates the "Structure Box" on the page.
<StructureSection load='2gnu' size='340' side='right'caption='[[2gnu]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2gnu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GNU FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
{{STRUCTURE_2gnu|  PDB=2gnu  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gnu OCA], [https://pdbe.org/2gnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gnu RCSB], [https://www.ebi.ac.uk/pdbsum/2gnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gnu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RCEH_CERSP RCEH_CERSP] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/2gnu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gnu ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bicontinuous lipidic cubic phases can be used as a host for growing crystals of membrane proteins. Since the cubic phase is stiff, handling is difficult and time-consuming. Moreover, the conventional cubic phase may interfere with the hydrophilic domains of membrane proteins due to the limited size of the aqueous pores. Here, we introduce a new crystallization method that makes use of a liquid analogue of the cubic phase, the sponge phase. This phase facilitates a considerable increase in the allowed size of aqueous domains of membrane proteins, and is easily generalised to a conventional vapour diffusion crystallisation experiment, including the use of nanoliter drop crystallization robots. The appearance of the sponge phase was confirmed by visual inspection, small-angle X-ray scattering and NMR spectroscopy. Crystals of the reaction centre from Rhodobacter sphaeroides were obtained by a conventional hanging-drop experiment, were harvested directly without the addition of lipase or cryoprotectant, and the structure was refined to 2.2 Angstroms resolution. In contrast to our earlier lipidic cubic phase reaction centre structure, the mobile ubiquinone could be built and refined. The practical advantages of the sponge phase make it a potent tool for crystallization of membrane proteins.


===The crystallization of reaction center from Rhodobacter sphaeroides occurs via a new route===
Lipidic sponge phase crystallization of membrane proteins.,Wadsten P, Wohri AB, Snijder A, Katona G, Gardiner AT, Cogdell RJ, Neutze R, Engstrom S J Mol Biol. 2006 Nov 17;364(1):44-53. Epub 2006 Jul 7. PMID:17005199<ref>PMID:17005199</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_17005199}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2gnu" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 17005199 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_17005199}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Cereibacter sphaeroides]]
2GNU is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GNU OCA].
[[Category: Large Structures]]
 
[[Category: Cogdell RJ]]
==Reference==
[[Category: Engstroem S]]
<ref group="xtra">PMID:17005199</ref><references group="xtra"/>
[[Category: Gardiner AT]]
[[Category: Rhodobacter sphaeroides]]
[[Category: Katona G]]
[[Category: Cogdell, R J.]]
[[Category: Neutze R]]
[[Category: Engstroem, S.]]
[[Category: Snijder A]]
[[Category: Gardiner, A T.]]
[[Category: Wadsten P]]
[[Category: Katona, G.]]
[[Category: Woehri AB]]
[[Category: Neutze, R.]]
[[Category: Snijder, A.]]
[[Category: Wadsten, P.]]
[[Category: Woehri, A B.]]
[[Category: Reaction centre]]
[[Category: Ubiquinone b]]
 
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