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New page: left|200px<br /><applet load="1z5u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z5u, resolution 2.3Å" /> '''Crystal structure of ...
 
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[[Image:1z5u.gif|left|200px]]<br /><applet load="1z5u" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1z5u, resolution 2.3&Aring;" />
'''Crystal structure of S. typhimurium AphA complexed with cyclic-AMP'''<br />


==Overview==
==Crystal structure of S. typhimurium AphA complexed with cyclic-AMP==
The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was, cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA, protein was purified to homogeneity. The protein crystallized in the, orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a =, 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent, content of approximately 54%. The crystals are composed of biologically, active AphA molecules.
<StructureSection load='1z5u' size='340' side='right'caption='[[1z5u]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1z5u]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z5U FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z5u OCA], [https://pdbe.org/1z5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z5u RCSB], [https://www.ebi.ac.uk/pdbsum/1z5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z5u ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/APHA_SALTM APHA_SALTM] Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (Probable).<ref>PMID:14501135</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z5/1z5u_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z5u ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1Z5U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with MG and CMP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z5U OCA].
*[[Acid phosphatase 3D structures|Acid phosphatase 3D structures]]
 
== References ==
==Reference==
<references/>
Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium., Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14501135 14501135]
__TOC__
[[Category: Acid phosphatase]]
</StructureSection>
[[Category: Salmonella typhimurium]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
[[Category: Kumar, V.]]
[[Category: Kumar V]]
[[Category: Makde, R.D.]]
[[Category: Makde RD]]
[[Category: CMP]]
[[Category: MG]]
[[Category: class-b bacterial acid phosphatase]]
[[Category: cyclic-amp complex of apha protein]]
[[Category: metalloenzyme]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:15:25 2007''

Latest revision as of 08:14, 25 October 2023

Crystal structure of S. typhimurium AphA complexed with cyclic-AMP

1z5u, resolution 2.30Å

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