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New page: left|200px<br /><applet load="1z96" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z96, resolution 1.80Å" /> '''Crystal structure of...
 
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[[Image:1z96.gif|left|200px]]<br /><applet load="1z96" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1z96, resolution 1.80&Aring;" />
'''Crystal structure of the Mud1 UBA domain'''<br />


==Overview==
==Crystal structure of the Mud1 UBA domain==
The ubiquitin-pathway associated (UBA) domain is a 40-residue, polyubiquitin-binding motif. The Schizosaccharomyces pombe protein Mud1 is, an ortholog of the Saccharomyces cerevisiae DNA-damage response protein, Ddi1 and binds to K48-linked polyubiquitin through its UBA domain. We have, solved the crystal structure of Mud1 UBA at 1.8 angstroms resolution, revealing a canonical three-helical UBA fold. We have probed the, interactions of this domain using mutagenesis, surface plasmon resonance, NMR and analytical ultracentrifugation. We show that the ubiquitin-binding, surface of Mud1 UBA extends beyond previously recognized motifs and can be, functionally dissected into primary and secondary ubiquitin-binding sites., Mutation of Phe330 to alanine, a residue exposed between helices 2 and 3, significantly reduces the affinity of the Mud1 UBA domain for K48-linked, polyubiquitin, despite leaving the primary binding surface functionally, intact. Moreover, K48-linked diubiquitin binds a single Mud1 UBA domain, even in the presence of excess UBA. We therefore propose a mechanism for, the recognition of K48-linked polyubiquitin chains by Mud1 in which, diubiquitin units are specifically recognized by a single UBA domain.
<StructureSection load='1z96' size='340' side='right'caption='[[1z96]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1z96]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z96 FirstGlance]. <br>
1Z96 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z96 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z96 OCA], [https://pdbe.org/1z96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z96 RCSB], [https://www.ebi.ac.uk/pdbsum/1z96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z96 ProSAT]</span></td></tr>
==Reference==
</table>
Mechanism of Lys48-linked polyubiquitin chain recognition by the Mud1 UBA domain., Trempe JF, Brown NR, Lowe ED, Gordon C, Campbell ID, Noble ME, Endicott JA, EMBO J. 2005 Sep 21;24(18):3178-89. Epub 2005 Sep 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16138082 16138082]
== Function ==
[https://www.uniprot.org/uniprot/MUD1_SCHPO MUD1_SCHPO]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Single protein]]
[[Category: Brown NR]]
[[Category: Brown, N.R.]]
[[Category: Campbell ID]]
[[Category: Campbell, I.D.]]
[[Category: Endicott JA]]
[[Category: Endicott, J.A.]]
[[Category: Gordon C]]
[[Category: Gordon, C.]]
[[Category: Johnson LN]]
[[Category: Johnson, L.N.]]
[[Category: Lowe ED]]
[[Category: Lowe, E.D.]]
[[Category: Noble MEM]]
[[Category: Noble, M.E.M.]]
[[Category: Trempe J-F]]
[[Category: Trempe, J.F.]]
[[Category: three-helix bundle]]
[[Category: uba]]
[[Category: ubiquitin]]
 
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