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New page: left|200px<br /><applet load="1zbx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zbx, resolution 2.5Å" /> '''Crystal structure of ...
 
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[[Image:1zbx.gif|left|200px]]<br /><applet load="1zbx" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1zbx, resolution 2.5&Aring;" />
'''Crystal structure of a Orc1p-Sir1p complex'''<br />


==Overview==
==Crystal structure of a Orc1p-Sir1p complex==
The interaction between silence information regulator 1 protein (Sir1p), and origin recognition complex 1 protein (Orc1p), the largest subunit of, the origin recognition complex, plays an important role in the, establishment of transcriptional silencing at the cryptic mating-type gene, loci in Saccharomyces cerevisiae. Sir1p binds the N-terminal region of, Orc1p encompassing a Bromo-adjacent homology (BAH) domain found in various, chromatin-associated proteins. To understand the molecular mechanism of, Sir protein recruitment, we have determined a 2.5-A cocrystal structure of, the N-terminal domain of Orc1p in complex with the Orc1p-interacting, domain of Sir1p. The structure reveals that Sir1p Orc1p-interacting domain, has a bilobal structure: an alpha/beta N-terminal lobe and a C-terminal, lobe resembling the Tudor domain royal family fold. The N-terminal lobe of, Sir1p binds in a shallow groove between a helical subdomain and the BAH, domain of Orc1p. The structure provides a mechanistic understanding of, Orc1p-Sir1p interaction specificity, as well as insights into, protein-protein interactions involving BAH domains in general.
<StructureSection load='1zbx' size='340' side='right'caption='[[1zbx]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1zbx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZBX FirstGlance]. <br>
1ZBX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZBX OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zbx OCA], [https://pdbe.org/1zbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zbx RCSB], [https://www.ebi.ac.uk/pdbsum/1zbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zbx ProSAT]</span></td></tr>
==Reference==
</table>
Structural basis for origin recognition complex 1 protein-silence information regulator 1 protein interaction in epigenetic silencing., Hsu HC, Stillman B, Xu RM, Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8519-24. Epub 2005 Jun 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15937111 15937111]
== Function ==
[[Category: Protein complex]]
[https://www.uniprot.org/uniprot/ORC1_YEAST ORC1_YEAST] Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.<ref>PMID:17825064</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zb/1zbx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zbx ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Hsu, H.C.]]
[[Category: Hsu HC]]
[[Category: Stillman, B.]]
[[Category: Stillman B]]
[[Category: Xu, R.M.]]
[[Category: Xu RM]]
[[Category: epigenetics]]
[[Category: protein-protein interaction]]
[[Category: silencing]]
[[Category: silent information regulators]]
 
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Retrieved from "https://proteopedia.org/1zbx"