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New page: left|200px<br /><applet load="1zhh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zhh, resolution 1.94Å" /> '''Crystal Structure of...
 
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[[Image:1zhh.gif|left|200px]]<br /><applet load="1zhh" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1zhh, resolution 1.94&Aring;" />
'''Crystal Structure of the Apo Form of Vibrio Harveyi LUXP Complexed with the Periplasmic Domain of LUXQ'''<br />


==Overview==
==Crystal Structure of the Apo Form of Vibrio Harveyi LUXP Complexed with the Periplasmic Domain of LUXQ==
The extracellular signaling molecule autoinducer-2 (AI-2) mediates, quorum-sensing communication in diverse bacterial species. In marine, vibrios, binding of AI-2 to the periplasmic receptor LuxP modulates the, activity of the inner membrane sensor kinase LuxQ, transducing the AI-2, information into the cytoplasm. Here, we show that Vibrio harveyi LuxP, associates with LuxQ in both the presence and absence of AI-2. The 1.9 A, X-ray crystal structure of apoLuxP, complexed with the periplasmic domain, of LuxQ, reveals that the latter contains two tandem, Per/ARNT/Simple-minded (PAS) folds. Thus, although many prokaryotic PAS, folds themselves bind ligands, the LuxQ periplasmic PAS folds instead bind, LuxP, monitoring its AI-2 occupancy. Mutations that disrupt the, apoLuxP:LuxQ interface sensitize V. harveyi to AI-2, implying that AI-2, binding causes the replacement of one set of LuxP:LuxQ contacts with, another. These conformational changes switch LuxQ between two opposing, enzymatic activities, each of which conveys information to the cytoplasm, about the cell density of the surrounding environment.
<StructureSection load='1zhh' size='340' side='right'caption='[[1zhh]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1zhh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZHH FirstGlance]. <br>
1ZHH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi] with NHE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZHH OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zhh OCA], [https://pdbe.org/1zhh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zhh RCSB], [https://www.ebi.ac.uk/pdbsum/1zhh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zhh ProSAT]</span></td></tr>
Regulation of LuxPQ receptor activity by the quorum-sensing signal autoinducer-2., Neiditch MB, Federle MJ, Miller ST, Bassler BL, Hughson FM, Mol Cell. 2005 May 27;18(5):507-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15916958 15916958]
</table>
[[Category: Protein complex]]
== Function ==
[https://www.uniprot.org/uniprot/LUXP_VIBHA LUXP_VIBHA] Binds to the signaling molecule autoinducer 2 (AI-2), a furanosyl borate diester, (3a-methyl-5,6-dihydrofuro-[2,3d][1,3,2]dioxaborole-2,2,6,6a-tetraol). This complex then interacts with the LuxQ sensor protein.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zh/1zhh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zhh ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio harveyi]]
[[Category: Vibrio harveyi]]
[[Category: Bassler, B.L.]]
[[Category: Bassler BL]]
[[Category: Federle, M.J.]]
[[Category: Federle MJ]]
[[Category: Hughson, F.M.]]
[[Category: Hughson FM]]
[[Category: Miller, S.T.]]
[[Category: Miller ST]]
[[Category: Neiditch, M.B.]]
[[Category: Neiditch MB]]
[[Category: NHE]]
[[Category: autoinducer-2 (ai-2)]]
[[Category: per/arnt/simple-minded (pas) fold]]
[[Category: periplasmic binding protein]]
[[Category: quorum sensing]]
[[Category: sensor kinase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:27:23 2007''
Retrieved from "https://proteopedia.org/1zhh"