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New page: left|200px<br /><applet load="1zie" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zie, resolution 1.44Å" /> '''Hydrogenated gammaE ...
 
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[[Image:1zie.gif|left|200px]]<br /><applet load="1zie" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1zie, resolution 1.44&Aring;" />
'''Hydrogenated gammaE crystallin in D2O solvent'''<br />


==Overview==
==Hydrogenated gammaE crystallin in D2O solvent==
Rat gammaE-crystallin was overexpressed, purified under different, labelling conditions and crystallized and X-ray data were collected at, resolutions between 1.71 and 1.36 A. The structures were determined by, molecular replacement. In these structures, the cd loop of the Greek-key, motif 3, which is the major structural key motif of the two, phase-transition groups of gamma-crystallins, presents a double, conformation. The influence of the perdeuteration on the protein structure, was determined by comparison of the atomic positions and temperature, factors of the different models. The perdeuterated proteins have a similar, structure to their hydrogenated counterparts, but partial or full, deuteration may have some effect on the atomic B-factor values.
<StructureSection load='1zie' size='340' side='right'caption='[[1zie]], [[Resolution|resolution]] 1.44&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1zie]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZIE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZIE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.44&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zie OCA], [https://pdbe.org/1zie PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zie RCSB], [https://www.ebi.ac.uk/pdbsum/1zie PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zie ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CRGE_RAT CRGE_RAT] Crystallins are the dominant structural components of the vertebrate eye lens.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zi/1zie_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zie ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Rat gammaE-crystallin was overexpressed, purified under different labelling conditions and crystallized and X-ray data were collected at resolutions between 1.71 and 1.36 A. The structures were determined by molecular replacement. In these structures, the cd loop of the Greek-key motif 3, which is the major structural key motif of the two phase-transition groups of gamma-crystallins, presents a double conformation. The influence of the perdeuteration on the protein structure was determined by comparison of the atomic positions and temperature factors of the different models. The perdeuterated proteins have a similar structure to their hydrogenated counterparts, but partial or full deuteration may have some effect on the atomic B-factor values.


==About this Structure==
A comparison of refined X-ray structures of hydrogenated and perdeuterated rat gammaE-crystallin in H2O and D2O.,Artero JB, Hartlein M, McSweeney S, Timmins P Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1541-9. Epub 2005, Oct 19. PMID:16239733<ref>PMID:16239733</ref>
1ZIE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with ACT and DOD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZIE OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
A comparison of refined X-ray structures of hydrogenated and perdeuterated rat gammaE-crystallin in H2O and D2O., Artero JB, Hartlein M, McSweeney S, Timmins P, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1541-9. Epub 2005, Oct 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16239733 16239733]
</div>
<div class="pdbe-citations 1zie" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Crystallin 3D structures|Crystallin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Artero JB]]
[[Category: Artero, J.B.]]
[[Category: Hartlein M]]
[[Category: Hartlein, M.]]
[[Category: McSweeney S]]
[[Category: McSweeney, S.]]
[[Category: Timmins P]]
[[Category: Timmins, P.]]
[[Category: ACT]]
[[Category: DOD]]
[[Category: 4 greek key motifs]]
 
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