1zik: Difference between revisions

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New page: left|200px<br /><applet load="1zik" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zik, resolution 1.8Å" /> '''GCN4-LEUCINE ZIPPER C...
 
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[[Image:1zik.gif|left|200px]]<br /><applet load="1zik" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1zik, resolution 1.8&Aring;" />
'''GCN4-LEUCINE ZIPPER CORE MUTANT ASN16LYS IN THE DIMERIC STATE'''<br />


==Overview==
==GCN4-LEUCINE ZIPPER CORE MUTANT ASN16LYS IN THE DIMERIC STATE==
A conserved asparagine (Asn 16) buried in the interface of the GCN4, leucine zipper selectively favours the parallel, dimeric, coiled-coil, structure. To test if other polar residues confer oligomerization, specificity, the structural effects of Gln and Lys substitutions for Asn, 16 were characterized. Like the wild-type peptide, the Asn 16Lys mutant, formed exclusively dimers. In contrast, Gln 16, despite its chemical, similarity to Asn, allowed the peptide to form both dimers and trimers., The Gln 16 side chain was accommodated by qualitatively different, interactions in the dimer and trimer crystal structures. These findings, demonstrate that the structural selectivity of polar residues results not, only from the burial of polar atoms, but also depends on the, complementarity of the side-chain stereochemistry with the surrounding, structural environment.
<StructureSection load='1zik' size='340' side='right'caption='[[1zik]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1zik]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZIK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zik OCA], [https://pdbe.org/1zik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zik RCSB], [https://www.ebi.ac.uk/pdbsum/1zik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zik ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.


==About this Structure==
==See Also==
1ZIK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZIK OCA].
*[[Gcn4 3D Structures|Gcn4 3D Structures]]
 
*[[Gnc4 3D Structures|Gnc4 3D Structures]]
==Reference==
__TOC__
Buried polar residues and structural specificity in the GCN4 leucine zipper., Gonzalez L Jr, Woolfson DN, Alber T, Nat Struct Biol. 1996 Dec;3(12):1011-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8946854 8946854]
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Alber T]]
[[Category: Alber, T.]]
[[Category: Gonzalez Junior L]]
[[Category: Junior, L.Gonzalez.]]
[[Category: Woolfson DN]]
[[Category: Woolfson, D.N.]]
[[Category: activator]]
[[Category: amino-acid biosynthesis]]
[[Category: coiled coil]]
[[Category: dna-binding]]
[[Category: leucine zipper]]
[[Category: nuclear protein]]
[[Category: transcription regulation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:28:37 2007''

Latest revision as of 09:05, 14 February 2024

GCN4-LEUCINE ZIPPER CORE MUTANT ASN16LYS IN THE DIMERIC STATE

1zik, resolution 1.80Å

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