1kix: Difference between revisions

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{{Seed}}
[[Image:1kix.png|left|200px]]


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==Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA==
The line below this paragraph, containing "STRUCTURE_1kix", creates the "Structure Box" on the page.
<StructureSection load='1kix' size='340' side='right'caption='[[1kix]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1kix]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KIX FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_1kix|  PDB=1kix  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kix OCA], [https://pdbe.org/1kix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kix RCSB], [https://www.ebi.ac.uk/pdbsum/1kix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kix ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TEBA_STENO TEBA_STENO] May function as protective capping of the single-stranded telomeric overhang. May also participate in telomere length regulation during DNA replication. Binds specifically to the T4G4-containing extension on the 3'strand and protects this region of the telomere from nuclease digestion and chemical modification.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ki/1kix_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kix ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Telomeres are the specialized protein--DNA complexes that cap and protect the ends of linear eukaryotic chromosomes. The extreme 3' end of the telomeric DNA in Oxytricha nova is bound by a two-subunit sequence-specific and 3' end-specific protein called the telomere end-binding protein (OnTEBP). Here we describe the crystal structure of the alpha-subunit of OnTEBP in complex with T4G4 single-stranded telomeric DNA. This structure shows an (alpha--ssDNA)2 homodimer with a large approximately 7,000 A2 protein--protein interface in which the domains of alpha are rearranged extensively from their positions in the structure of an alpha--beta--ssDNA ternary complex. The (alpha--ssDNA)2 complex can bind two telomeres on opposite sides of the dimer and, thus, acts as a protein mediator of telomere--telomere associations. The structures of the (alpha--ssDNA)2 dimer presented here and the previously described alpha--beta--ssDNA complex demonstrate that OnTEBP forms multiple telomeric complexes that potentially mediate the assembly and disassembly of higher order telomeric structures.


===Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA===
Dimeric structure of the Oxytricha nova telomere end-binding protein alpha-subunit bound to ssDNA.,Peersen OB, Ruggles JA, Schultz SC Nat Struct Biol. 2002 Mar;9(3):182-7. PMID:11836536<ref>PMID:11836536</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1kix" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_11836536}}, adds the Publication Abstract to the page
*[[End-binding protein|End-binding protein]]
(as it appears on PubMed at http://www.pubmed.gov), where 11836536 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_11836536}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
1KIX is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIX OCA].
 
==Reference==
<ref group="xtra">PMID:11836536</ref><references group="xtra"/>
[[Category: Sterkiella nova]]
[[Category: Sterkiella nova]]
[[Category: Peersen, O B.]]
[[Category: Peersen OB]]
[[Category: Ruggles, J A.]]
[[Category: Ruggles JA]]
[[Category: Schultz, S C.]]
[[Category: Schultz SC]]
[[Category: Dna-protein interaction]]
[[Category: Single stranded dna]]
[[Category: Ssdna]]
[[Category: Telomere binding protein]]
 
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