1zn1: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
New page: left|200px<br /><applet load="1zn1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zn1" /> '''Coordinates of RRF fitted into Cryo-EM map o...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(20 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1zn1.gif|left|200px]]<br /><applet load="1zn1" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1zn1" />
'''Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex'''<br />


==Overview==
==Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex==
Ribosome recycling, the disassembly of the posttermination complex after, each round of protein synthesis, is an essential step in mRNA translation, but its mechanism has remained obscure. In eubacteria, recycling is, catalyzed by RRF (ribosome recycling factor) and EF-G (elongation factor, G). By using cryo-electron microscopy, we have obtained two density maps, one of the RRF bound posttermination complex and one of the 50S subunit, bound with both EF-G and RRF. Comparing the two maps, we found domain I of, RRF to be in the same orientation, while domain II in the EF-G-containing, 50S subunit is extensively rotated (approximately 60 degrees) compared to, its orientation in the 70S complex. Mapping the 50S conformation of RRF, onto the 70S posttermination complex suggests that it can disrupt the, intersubunit bridges B2a and B3, and thus effect a separation of the two, subunits. These observations provide the structural basis for the, mechanism by which the posttermination complex is split into subunits by, the joint action of RRF and EF-G.
<SX load='1zn1' size='340' side='right' viewer='molstar' caption='[[1zn1]], [[Resolution|resolution]] 14.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1zn1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZN1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 14.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zn1 OCA], [https://pdbe.org/1zn1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zn1 RCSB], [https://www.ebi.ac.uk/pdbsum/1zn1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zn1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RRF_ECOLI RRF_ECOLI] Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.[HAMAP-Rule:MF_00040]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zn/1zn1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zn1 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1ZN1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZN1 OCA].
*[[Ribosomal protein S12|Ribosomal protein S12]]
 
*[[Ribosome recycling factor|Ribosome recycling factor]]
==Reference==
__TOC__
Mechanism for the disassembly of the posttermination complex inferred from cryo-EM studies., Gao N, Zavialov AV, Li W, Sengupta J, Valle M, Gursky RP, Ehrenberg M, Frank J, Mol Cell. 2005 Jun 10;18(6):663-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15949441 15949441]
</SX>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Ehrenberg, M.]]
[[Category: Ehrenberg M]]
[[Category: Frank, J.]]
[[Category: Frank J]]
[[Category: Gao, N.]]
[[Category: Gao N]]
[[Category: Gursky, R.P.]]
[[Category: Gursky RP]]
[[Category: Li, W.]]
[[Category: Li W]]
[[Category: Sengupta, J.]]
[[Category: Sengupta J]]
[[Category: Valle, M.]]
[[Category: Valle M]]
[[Category: Zavialov, A.V.]]
[[Category: Zavialov AV]]
[[Category: 70s]]
[[Category: elongation factor g]]
[[Category: post-termination complex]]
[[Category: ribosome recycling factor]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:32:46 2007''

Latest revision as of 09:05, 14 February 2024

Coordinates of RRF fitted into Cryo-EM map of the 70S post-termination complex

Loading...

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1zn1&oldid=4057296"