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New page: left|200px<br /><applet load="1zp2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zp2, resolution 3.0Å" /> '''Structure of the Medi...
 
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[[Image:1zp2.gif|left|200px]]<br /><applet load="1zp2" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1zp2, resolution 3.0&Aring;" />
'''Structure of the Mediator subunit cyclin C'''<br />


==Overview==
==Structure of the Mediator subunit cyclin C==
Cyclin C binds the cyclin-dependent kinases CDK8 and CDK3, which regulate, mRNA transcription and the cell cycle, respectively. The crystal structure, of cyclin C reveals two canonical five-helix repeats and a specific, N-terminal helix. In contrast to other cyclins, the N-terminal helix is, short, mobile, and in an exposed position that allows for interactions, with proteins other than the CDKs. A model of the CDK8/cyclin C pair, reveals two regions in the interface with apparently distinct roles. A, conserved region explains promiscuous binding of cyclin C to CDK8 and, CDK3, and a non-conserved region may be responsible for discrimination of, CDK8 against other CDKs involved in transcription. A conserved and cyclin, C-specific surface groove may recruit substrates near the CDK8 active, site. Activation of CDKs generally involves phosphorylation of a loop at a, threonine residue. In CDK8, this loop is longer and the threonine is, absent, suggesting an alternative mechanism of activation that we discuss, based on a CDK8-cyclin C model.
<StructureSection load='1zp2' size='340' side='right'caption='[[1zp2]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1zp2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZP2 FirstGlance]. <br>
1ZP2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZP2 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zp2 OCA], [https://pdbe.org/1zp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zp2 RCSB], [https://www.ebi.ac.uk/pdbsum/1zp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zp2 ProSAT]</span></td></tr>
==Reference==
</table>
Structure of the mediator subunit cyclin C and its implications for CDK8 function., Hoeppner S, Baumli S, Cramer P, J Mol Biol. 2005 Jul 29;350(5):833-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15979093 15979093]
== Function ==
[https://www.uniprot.org/uniprot/SRB11_SCHPO SRB11_SCHPO] Component of the Cdk8 module/Srb8-11 module which is a regulatory module of the Mediator complex that regulates basal RNA polymerase II transcription. The Cdk8 module may sterically hinder the interaction between Mediator and RNA polymerase II leading to transcriptional repression of a subset of genes regulated by Mediator.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zp/1zp2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zp2 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Single protein]]
[[Category: Baumli S]]
[[Category: Baumli, S.]]
[[Category: Cramer P]]
[[Category: Cramer, P.]]
[[Category: Hoeppner S]]
[[Category: Hoeppner, S.]]
[[Category: cyclin repeat domains]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:34:59 2007''

Latest revision as of 09:06, 14 February 2024

Structure of the Mediator subunit cyclin C

1zp2, resolution 3.00Å

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