1afw: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px<br /> <applet load="1afw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1afw, resolution 1.8Å" /> '''THE 1.8 ANGSTROM CRY...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(22 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1afw.gif|left|200px]]<br />
<applet load="1afw" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1afw, resolution 1.8&Aring;" />
'''THE 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE DIMERIC PEROXISOMAL THIOLASE OF SACCHAROMYCES CEREVISIAE'''<br />


==Overview==
==THE 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE DIMERIC PEROXISOMAL THIOLASE OF SACCHAROMYCES CEREVISIAE==
The dimeric, peroxisomal 3-ketoacyl-CoA thiolase catalyses the conversion, of 3-ketoacyl-CoA into acyl-CoA, which is shorter by two carbon atoms., This reaction is the last step of the beta-oxidation pathway. The crystal, structure of unliganded peroxisomal thiolase of the yeast Saccharomyces, cerevisiae has been refined at 1.8 A resolution. An unusual feature of, this structure is the presence of two helices, completely buried in the, dimer and sandwiched between two beta-sheets. The analysis of the, structure shows that the sequences of these helices are not hydrophobic, but generate two amphipathic helices. The helix in the N-terminal domain, exposes the polar side-chains to a cavity at the dimer interface, filled, with structured water molecules. The central helix in the C-terminal, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9402066 (full description)]]
<StructureSection load='1afw' size='340' side='right'caption='[[1afw]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1afw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AFW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1afw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1afw OCA], [https://pdbe.org/1afw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1afw RCSB], [https://www.ebi.ac.uk/pdbsum/1afw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1afw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/THIK_YEAST THIK_YEAST]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/1afw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1afw ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1AFW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with MRD as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.16 2.3.1.16]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AFW OCA]].
*[[Thiolase 3D structures|Thiolase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The 1.8 A crystal structure of the dimeric peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: implications for substrate binding and reaction mechanism., Mathieu M, Modis Y, Zeelen JP, Engel CK, Abagyan RA, Ahlberg A, Rasmussen B, Lamzin VS, Kunau WH, Wierenga RK, J Mol Biol. 1997 Oct 31;273(3):714-28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9402066 9402066]
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Mathieu M]]
[[Category: Mathieu, M.]]
[[Category: Wierenga RK]]
[[Category: Wierenga, R.K.]]
[[Category: MRD]]
[[Category: fatty acid metabolism]]
[[Category: thiolase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 21:41:09 2007''
Retrieved from "https://proteopedia.org/1afw"