1v7x: Difference between revisions

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{{Seed}}
[[Image:1v7x.png|left|200px]]


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==Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate==
The line below this paragraph, containing "STRUCTURE_1v7x", creates the "Structure Box" on the page.
<StructureSection load='1v7x' size='340' side='right'caption='[[1v7x]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1v7x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_proteolyticus Vibrio proteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V7X FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_1v7x|  PDB=1v7x  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v7x OCA], [https://pdbe.org/1v7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v7x RCSB], [https://www.ebi.ac.uk/pdbsum/1v7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v7x ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CHBP_VIBPR CHBP_VIBPR] Catalyzes the reversible phosphorolysis of chitobiose (N,N'-diacetylchitobiose or (GlcNAc)(2)) into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration.<ref>PMID:13678418</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v7/1v7x_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v7x ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Vibrio proteolyticus chitobiose phosphorylase (ChBP) belongs to glycosyl transferase family 36 (GT-36), and catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. As the first known structures of a GT-36 enzyme, we determined the crystal structure of ChBP in a ternary complex with GlcNAc and SO(4). It is also the first structures of an inverting phosphorolytic enzyme in a complex with a sugar and a sulfate ion, and reveals a pseudo-ternary complex structure of enzyme-sugar-phosphate. ChBP comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain, constituting a distinctive structure among GT families. Instead, it shows significant structural similarity with glycoside hydrolase (GH) enzymes, glucoamylases (GH-15), and maltose phosphorylase (GH-65) in clan GH-L. The structural similarity reported here, together with distant sequence similarities between ChBP and GHs, led to the reclassification of family GT-36 into a novel GH family, namely GH-94.


===Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate===
Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold.,Hidaka M, Honda Y, Kitaoka M, Nirasawa S, Hayashi K, Wakagi T, Shoun H, Fushinobu S Structure. 2004 Jun;12(6):937-47. PMID:15274915<ref>PMID:15274915</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1v7x" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 15274915 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_15274915}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
1V7X is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_proteolyticus Vibrio proteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V7X OCA].
 
==Reference==
<ref group="xtra">PMID:15274915</ref><references group="xtra"/>
[[Category: Vibrio proteolyticus]]
[[Category: Vibrio proteolyticus]]
[[Category: Fushinobu, S.]]
[[Category: Fushinobu S]]
[[Category: Hayashi, K.]]
[[Category: Hayashi K]]
[[Category: Hidaka, M.]]
[[Category: Hidaka M]]
[[Category: Honda, Y.]]
[[Category: Honda Y]]
[[Category: Kitaoka, M.]]
[[Category: Kitaoka M]]
[[Category: Nirasawa, S.]]
[[Category: Nirasawa S]]
[[Category: Shoun, H.]]
[[Category: Shoun H]]
[[Category: Wakagi, T.]]
[[Category: Wakagi T]]
[[Category: Beta-sandwich]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:58:04 2009''
Retrieved from "https://proteopedia.org/1v7x"