2a2f: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px<br /><applet load="2a2f" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a2f, resolution 2.50Å" /> '''Crystal Structure of...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:2a2f.gif|left|200px]]<br /><applet load="2a2f" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2a2f, resolution 2.50&Aring;" />
'''Crystal Structure of Sec15 C-terminal domain'''<br />


==Overview==
==Crystal Structure of Sec15 C-terminal domain==
Sec15, a component of the exocyst, recognizes vesicle-associated Rab, GTPases, helps target transport vesicles to the budding sites in yeast and, is thought to recruit other exocyst proteins. Here we report the, characterization of a 35-kDa fragment that comprises most of the, C-terminal half of Drosophila melanogaster Sec15. This C-terminal domain, was found to bind a subset of Rab GTPases, especially Rab11, in a, GTP-dependent manner. We also provide evidence that in fly photoreceptors, Sec15 colocalizes with Rab11 and that loss of Sec15 affects rhabdomere, morphology. Determination of the 2.5-A crystal structure of the C-terminal, domain revealed a novel fold consisting of ten alpha-helices equally, distributed between two subdomains (N and C subdomains). We show that the, C subdomain, mainly via a single helix, is sufficient for Rab binding.
<StructureSection load='2a2f' size='340' side='right'caption='[[2a2f]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2a2f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A2F FirstGlance]. <br>
2A2F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A2F OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a2f OCA], [https://pdbe.org/2a2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a2f RCSB], [https://www.ebi.ac.uk/pdbsum/2a2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a2f ProSAT]</span></td></tr>
==Reference==
</table>
Sec15 interacts with Rab11 via a novel domain and affects Rab11 localization in vivo., Wu S, Mehta SQ, Pichaud F, Bellen HJ, Quiocho FA, Nat Struct Mol Biol. 2005 Oct;12(10):879-85. Epub 2005 Sep 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16155582 16155582]
== Function ==
[https://www.uniprot.org/uniprot/EXOC6_DROME EXOC6_DROME] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a2/2a2f_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a2f ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bellen, H.J.]]
[[Category: Bellen HJ]]
[[Category: Mehta, S.Q.]]
[[Category: Mehta SQ]]
[[Category: Pichaud, F.]]
[[Category: Pichaud F]]
[[Category: Quiocho, F.A.]]
[[Category: Quiocho FA]]
[[Category: Wu, S.]]
[[Category: Wu S]]
[[Category: all helical structure]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:52:51 2007''

Latest revision as of 09:10, 14 February 2024

Crystal Structure of Sec15 C-terminal domain

2a2f, resolution 2.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2a2f&oldid=4057594"