2pfb: Difference between revisions

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{{Seed}}
[[Image:2pfb.png|left|200px]]


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==Structure of oxidized OhrR from Xanthamonas campestris==
The line below this paragraph, containing "STRUCTURE_2pfb", creates the "Structure Box" on the page.
<StructureSection load='2pfb' size='340' side='right'caption='[[2pfb]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2pfb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PFB FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pfb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pfb OCA], [https://pdbe.org/2pfb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pfb RCSB], [https://www.ebi.ac.uk/pdbsum/2pfb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pfb ProSAT]</span></td></tr>
{{STRUCTURE_2pfb|  PDB=2pfb  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q93R11_XANCH Q93R11_XANCH]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pf/2pfb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pfb ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Xanthomonas campestris transcription regulator OhrR contains a reactive cysteine residue (C22) that upon oxidation by organic hydroperoxides (OHPs) forms an intersubunit disulphide bond with residue C127'. Such modification induces the expression of a peroxidase that reduces OHPs to their less toxic alcohols. Here, we describe the structures of reduced and OHP-oxidized OhrR, visualizing the structural mechanism of OHP induction. Reduced OhrR takes a canonical MarR family fold with C22 and C127' separated by 15.5 A. OHP oxidation results in the disruption of the Y36'-C22-Y47' interaction network and dissection of helix alpha5, which then allows the 135 degrees rotation and 8.2 A translation of C127', formation of the C22-C127' disulphide bond, and alpha6-alpha6' helix-swapped reconfiguration of the dimer interface. These changes result in the 28 degrees rigid body rotations of each winged helix-turn-helix motif and DNA dissociation. Similar effector-induced rigid body rotations are expected for most MarR family members.


===Structure of oxidized OhrR from Xanthamonas campestris===
Structural mechanism of organic hydroperoxide induction of the transcription regulator OhrR.,Newberry KJ, Fuangthong M, Panmanee W, Mongkolsuk S, Brennan RG Mol Cell. 2007 Nov 30;28(4):652-64. PMID:18042459<ref>PMID:18042459</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2pfb" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_18042459}}, adds the Publication Abstract to the page
*[[Organic hydroperoxide resistance protein|Organic hydroperoxide resistance protein]]
(as it appears on PubMed at http://www.pubmed.gov), where 18042459 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_18042459}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2PFB is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFB OCA].
 
==Reference==
<ref group="xtra">PMID:18042459</ref><references group="xtra"/>
[[Category: Xanthomonas campestris]]
[[Category: Xanthomonas campestris]]
[[Category: Brennan, R G.]]
[[Category: Brennan RG]]
[[Category: Newberry, K J.]]
[[Category: Newberry KJ]]
[[Category: Marr family]]
[[Category: Transcription regulator]]
[[Category: Transcriptional regulator]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 01:56:50 2009''

Latest revision as of 08:28, 30 October 2024

Structure of oxidized OhrR from Xanthamonas campestris

2pfb, resolution 1.93Å

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