1ft7: Difference between revisions

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{{Seed}}
[[Image:1ft7.png|left|200px]]


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==AAP COMPLEXED WITH L-LEUCINEPHOSPHONIC ACID==
The line below this paragraph, containing "STRUCTURE_1ft7", creates the "Structure Box" on the page.
<StructureSection load='1ft7' size='340' side='right'caption='[[1ft7]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1ft7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_proteolyticus Vibrio proteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FT7 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PLU:LEUCINE+PHOSPHONIC+ACID'>PLU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
{{STRUCTURE_1ft7|  PDB=1ft7  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ft7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ft7 OCA], [https://pdbe.org/1ft7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ft7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ft7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ft7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AMPX_VIBPR AMPX_VIBPR]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/1ft7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ft7 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The nature of the interaction of the transition-state analogue inhibitor L-leucinephosphonic acid (LPA) with the leucine aminopeptidase from Aeromonas proteolytica (AAP) was investigated. LPA was shown to be a competitive inhibitor at pH 8.0 with a K(i) of 6.6 microM. Electronic absorption spectra, recorded at pH 7.5 of [CoCo(AAP)], [CoZn(AAP)], and [ZnCo(AAP)] upon addition of LPA suggest that LPA interacts with both metal ions in the dinuclear active site. EPR studies on the Co(II)-substituted forms of AAP revealed that the environments of the Co(II) ions in both [CoZn(AAP)] and [ZnCo(AAP)] become highly asymmetric and constrained upon the addition of LPA and clearly indicate that LPA interacts with both metal ions. The X-ray crystal structure of AAP complexed with LPA was determined at 2.1 A resolution. The X-ray crystallographic data indicate that LPA interacts with both metal centers in the dinuclear active site of AAP and a single oxygen atom bridge is absent. Thus, LPA binds to the dinuclear active site of AAP as an eta-1,2-mu-phosphonate with one ligand to the second metal ion provided by the N-terminal amine. A structural comparison of the binding of phosphonate-containing transition-state analogues to the mono- and bimetallic peptidases provides insight into the requirement for the second metal ion in bridged bimetallic peptidases. On the basis of the results obtained from the spectroscopic and X-ray crystallographic data presented herein along with previously reported mechanistic data for AAP, a new catalytic mechanism for the hydrolysis reaction catalyzed by AAP is proposed.


===AAP COMPLEXED WITH L-LEUCINEPHOSPHONIC ACID===
Inhibition of the aminopeptidase from Aeromonas proteolytica by L-leucinephosphonic acid. Spectroscopic and crystallographic characterization of the transition state of peptide hydrolysis.,Stamper C, Bennett B, Edwards T, Holz RC, Ringe D, Petsko G Biochemistry. 2001 Jun 19;40(24):7035-46. PMID:11401547<ref>PMID:11401547</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1ft7" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_11401547}}, adds the Publication Abstract to the page
*[[Aminopeptidase 3D structures|Aminopeptidase 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 11401547 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_11401547}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
1FT7 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_proteolyticus Vibrio proteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FT7 OCA].
 
==Reference==
<ref group="xtra">PMID:11401547</ref><references group="xtra"/>
[[Category: Bacterial leucyl aminopeptidase]]
[[Category: Vibrio proteolyticus]]
[[Category: Vibrio proteolyticus]]
[[Category: Bennett, B.]]
[[Category: Bennett B]]
[[Category: Holz, R.]]
[[Category: Holz R]]
[[Category: Petsko, G.]]
[[Category: Petsko G]]
[[Category: Ringe, D.]]
[[Category: Ringe D]]
[[Category: Stamper, C.]]
[[Category: Stamper C]]
[[Category: Bimetallic]]
[[Category: Peptidase]]
[[Category: Zinc]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 03:41:12 2009''
Retrieved from "https://proteopedia.org/1ft7"