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New page: left|200px<br /><applet load="2aoz" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aoz, resolution 2.08Å" /> '''Crystal structure of...
 
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[[Image:2aoz.gif|left|200px]]<br /><applet load="2aoz" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2aoz, resolution 2.08&Aring;" />
'''Crystal structure of the myotoxin-II from Atropoides nummifer venom'''<br />


==Overview==
==Crystal structure of the myotoxin-II from Atropoides nummifer venom==
Lys49 snake-venom phospholipase A2 (PLA2) homologues are highly myotoxic, proteins which, although lacking catalytic activity, possess the ability, to disrupt biological membranes, inducing significant muscle-tissue loss, and permanent disability in severely envenomed patients. Since the, structural basis for their toxic activity is still only partially, understood, the structure of myotoxin II, a monomeric Lys49 PLA2 homologue, from Atropoides nummifer, has been determined at 2.08 angstroms resolution, and the anion-binding site has been characterized.
<StructureSection load='2aoz' size='340' side='right'caption='[[2aoz]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2aoz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Metlapilcoatlus_nummifer Metlapilcoatlus nummifer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AOZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AOZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.08&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aoz OCA], [https://pdbe.org/2aoz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aoz RCSB], [https://www.ebi.ac.uk/pdbsum/2aoz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aoz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PA2HB_METNM PA2HB_METNM] Snake venom phospholipase A2 homolog that lacks enzymatic activity, but has myotoxic and cytolytic activities.<ref>PMID:12127577</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ao/2aoz_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aoz ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lys49 snake-venom phospholipase A2 (PLA2) homologues are highly myotoxic proteins which, although lacking catalytic activity, possess the ability to disrupt biological membranes, inducing significant muscle-tissue loss and permanent disability in severely envenomed patients. Since the structural basis for their toxic activity is still only partially understood, the structure of myotoxin II, a monomeric Lys49 PLA2 homologue from Atropoides nummifer, has been determined at 2.08 angstroms resolution and the anion-binding site has been characterized.


==About this Structure==
Structure of myotoxin II, a catalytically inactive Lys49 phospholipase A2 homologue from Atropoides nummifer venom.,Murakami MT, Melo CC, Angulo Y, Lomonte B, Arni RK Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 May 1;62(Pt, 5):423-6. Epub 2006 Apr 12. PMID:16682766<ref>PMID:16682766</ref>
2AOZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Atropoides_nummifer Atropoides nummifer] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AOZ OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of myotoxin II, a catalytically inactive Lys49 phospholipase A2 homologue from Atropoides nummifer venom., Murakami MT, Melo CC, Angulo Y, Lomonte B, Arni RK, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 May 1;62(Pt, 5):423-6. Epub 2006 Apr 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16682766 16682766]
</div>
[[Category: Atropoides nummifer]]
<div class="pdbe-citations 2aoz" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
[[Category: Angulo, Y.]]
[[Category: Arni, R.K.]]
[[Category: Lomonte, B.]]
[[Category: Melo, C.C.]]
[[Category: Murakami, M.T.]]
[[Category: SO4]]
[[Category: atropoides nummifer]]
[[Category: myotoxicity]]
[[Category: phospholipase a2]]
[[Category: x-ray diffration]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:15:51 2007''
==See Also==
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
*[[Phospholipase A2 homolog|Phospholipase A2 homolog]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Metlapilcoatlus nummifer]]
[[Category: Angulo Y]]
[[Category: Arni RK]]
[[Category: Lomonte B]]
[[Category: Melo CC]]
[[Category: Murakami MT]]
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