2juo: Difference between revisions

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{{Seed}}
[[Image:2juo.png|left|200px]]


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==GABPa OST domain==
The line below this paragraph, containing "STRUCTURE_2juo", creates the "Structure Box" on the page.
<StructureSection load='2juo' size='340' side='right'caption='[[2juo]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2juo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JUO FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2juo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2juo OCA], [https://pdbe.org/2juo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2juo RCSB], [https://www.ebi.ac.uk/pdbsum/2juo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2juo ProSAT]</span></td></tr>
{{STRUCTURE_2juo|  PDB=2juo  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/GABPA_MOUSE GABPA_MOUSE] Transcription factor capable of interacting with purine rich repeats (GA repeats).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ju/2juo_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2juo ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Using NMR spectroscopy, we identified and characterized a previously unrecognized structured domain near the N-terminus (residues 35-121) of the ETS family transcription factor GABP alpha. The monomeric domain folds as a five-stranded beta-sheet crossed by a distorted helix. Although globally resembling ubiquitin, the GABP alpha fragment differs in its secondary structure topology and thus appears to represent a new protein fold that we term the OST (On-SighT) domain. The surface of the GABP alpha OST domain contains two predominant clusters of negatively-charged residues suggestive of electrostatically driven interactions with positively-charged partner proteins. Following a best-candidate approach to identify such a partner, we demonstrated through NMR-monitored titrations and glutathione S-transferase pulldown assays that the OST domain binds to the CH1 and CH3 domains of the co-activator histone acetyltransferase CBP/p300. This provides a direct structural link between GABP and a central component of the transcriptional machinery.


===GABPa OST domain===
Identification and structural characterization of a CBP/p300-binding domain from the ETS family transcription factor GABP alpha.,Kang HS, Nelson ML, Mackereth CD, Scharpf M, Graves BJ, McIntosh LP J Mol Biol. 2008 Mar 28;377(3):636-46. Epub 2008 Jan 30. PMID:18295234<ref>PMID:18295234</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2juo" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 18295234 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_18295234}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2JUO is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUO OCA].
 
==Reference==
<ref group="xtra">PMID:18295234</ref><references group="xtra"/>
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Graves, B J.]]
[[Category: Graves BJ]]
[[Category: Kang, H.]]
[[Category: Kang H]]
[[Category: Mackereth, C D.]]
[[Category: Mackereth CD]]
[[Category: McIntosh, L P.]]
[[Category: McIntosh LP]]
[[Category: Nelson, M L.]]
[[Category: Nelson ML]]
[[Category: Schaerpf, M.]]
[[Category: Schaerpf M]]
[[Category: Dna-binding]]
[[Category: Ga-binding protein]]
[[Category: Nmr ensemble]]
[[Category: Nucleus]]
[[Category: Ost]]
[[Category: Transcription factor]]
[[Category: Transcription regulation]]
[[Category: Ubiquitin]]
 
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Retrieved from "https://proteopedia.org/2juo"