2b9l: Difference between revisions

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{{Seed}}
[[Image:2b9l.png|left|200px]]


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==Crystal structure of prophenoloxidase activating factor-II from the beetle Holotrichia diomphalia==
The line below this paragraph, containing "STRUCTURE_2b9l", creates the "Structure Box" on the page.
<StructureSection load='2b9l' size='340' side='right'caption='[[2b9l]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2b9l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Holotrichia_diomphalia Holotrichia diomphalia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B9L FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_2b9l|  PDB=2b9l  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b9l OCA], [https://pdbe.org/2b9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b9l RCSB], [https://www.ebi.ac.uk/pdbsum/2b9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b9l ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PPAF2_HOLDI PPAF2_HOLDI] Binds and activates processed prophenoloxidases PPO1 and PPO2 and thus is involved in the activation of the prophenoloxidase cascade probably following the recognition of pathogen-derived products.<ref>PMID:11012672</ref> <ref>PMID:12185078</ref> <ref>PMID:16362048</ref> <ref>PMID:9652393</ref> <ref>PMID:12185078</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b9/2b9l_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b9l ConSurf].
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== Publication Abstract from PubMed ==
Clip-domain serine proteases (SPs) are the essential components of extracellular signaling cascades in various biological processes, especially in embryonic development and the innate immune responses of invertebrates. They consist of a chymotrypsin-like SP domain and one or two clip domains at the N-terminus. Prophenoloxidase-activating factor (PPAF)-II, which belongs to the noncatalytic clip-domain SP family, is indispensable for the generation of the active phenoloxidase leading to melanization, a major defense mechanism of insects. Here, the crystal structure of PPAF-II reveals that the clip domain adopts a novel fold containing a central cleft, which is distinct from the structures of defensins with a similar arrangement of cysteine residues. Ensuing studies demonstrated that PPAF-II forms a homo-oligomer upon cleavage by the upstream protease and that the clip domain of PPAF-II functions as a module for binding phenoloxidase through the central cleft, while the clip domain of a catalytically active easter-type SP plays an essential role in the rapid activation of its protease domain.


===Crystal structure of prophenoloxidase activating factor-II from the beetle Holotrichia diomphalia===
Crystal structure of a clip-domain serine protease and functional roles of the clip domains.,Piao S, Song YL, Kim JH, Park SY, Park JW, Lee BL, Oh BH, Ha NC EMBO J. 2005 Dec 21;24(24):4404-14. Epub 2005 Dec 15. PMID:16362048<ref>PMID:16362048</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2b9l" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 16362048 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_16362048}}
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</StructureSection>
==About this Structure==
2B9L is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Holotrichia_diomphalia Holotrichia diomphalia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9L OCA].
 
==Reference==
<ref group="xtra">PMID:16362048</ref><references group="xtra"/>
[[Category: Holotrichia diomphalia]]
[[Category: Holotrichia diomphalia]]
[[Category: Ha, N C.]]
[[Category: Large Structures]]
[[Category: Lee, B L.]]
[[Category: Ha N-C]]
[[Category: Oh, B H.]]
[[Category: Lee BL]]
[[Category: Park, S Y.]]
[[Category: Oh B-H]]
[[Category: Piao, S.]]
[[Category: Park SY]]
[[Category: Song, Y L.]]
[[Category: Piao S]]
[[Category: Clip domain]]
[[Category: Song Y-L]]
[[Category: Easter]]
[[Category: Innate immunity]]
[[Category: Melanin]]
 
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Retrieved from "https://proteopedia.org/2b9l"