2zf8: Difference between revisions

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{{Seed}}
[[Image:2zf8.png|left|200px]]


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==Crystal structure of MotY==
The line below this paragraph, containing "STRUCTURE_2zf8", creates the "Structure Box" on the page.
<StructureSection load='2zf8' size='340' side='right'caption='[[2zf8]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2zf8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_alginolyticus Vibrio alginolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZF8 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zf8 OCA], [https://pdbe.org/2zf8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zf8 RCSB], [https://www.ebi.ac.uk/pdbsum/2zf8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zf8 ProSAT]</span></td></tr>
{{STRUCTURE_2zf8|  PDB=2zf8  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/P74944_VIBAL P74944_VIBAL]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zf/2zf8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zf8 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Rotation of the sodium-driven polar flagellum of Vibrio alginolyticus requires four motor proteins: PomA, PomB, MotX, and MotY. PomA and PomB form a sodium-ion channel in the cytoplasmic membrane that functions as a stator complex to couple sodium-ion flux with torque generation. MotX and MotY are components of the T-ring, which is located beneath the P-ring of the polar flagellar basal body and is involved in incorporation of the PomA/PomB complex into the motor. Here, we describe the determination of the crystal structure of MotY at 2.9 A resolution. The structure shows two distinct domains: an N-terminal domain (MotY-N) and a C-terminal domain (MotY-C). MotY-N has a unique structure. MotY-C contains a putative peptidoglycan-binding motif that is remarkably similar to those of peptidoglycan-binding proteins, such as Pal and RmpM, but this region is disordered in MotY. Motility assay of cells producing either of the MotY-N and MotY-C fragments and subsequent biochemical analyses indicate that MotY-N is essential for association of the stator units around the rotor, whereas MotY-C stabilizes the association by binding to the peptidoglycan layer. Based on these observations, we propose a model for the mechanism of stator assembly around the rotor.


===Crystal structure of MotY===
Insights into the stator assembly of the Vibrio flagellar motor from the crystal structure of MotY.,Kojima S, Shinohara A, Terashima H, Yakushi T, Sakuma M, Homma M, Namba K, Imada K Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7696-701. Epub 2008 May 27. PMID:18505842<ref>PMID:18505842</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_18505842}}, adds the Publication Abstract to the page
<div class="pdbe-citations 2zf8" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 18505842 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_18505842}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2ZF8 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_alginolyticus Vibrio alginolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZF8 OCA].
 
==Reference==
<ref group="xtra">PMID:18505842</ref><references group="xtra"/>
[[Category: Vibrio alginolyticus]]
[[Category: Vibrio alginolyticus]]
[[Category: Homma, M.]]
[[Category: Homma M]]
[[Category: Imada, K.]]
[[Category: Imada K]]
[[Category: Kojima, S.]]
[[Category: Kojima S]]
[[Category: Namba, K.]]
[[Category: Namba K]]
[[Category: 2-layer sandwich]]
[[Category: Beta barrel]]
[[Category: Flagellum]]
[[Category: Structural protein]]
 
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Retrieved from "https://proteopedia.org/2zf8"