2hl7: Difference between revisions

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{{Seed}}
[[Image:2hl7.png|left|200px]]


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==Crystal structure of the periplasmic domain of CcmH from Pseudomonas aeruginosa==
The line below this paragraph, containing "STRUCTURE_2hl7", creates the "Structure Box" on the page.
<StructureSection load='2hl7' size='340' side='right'caption='[[2hl7]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2hl7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HL7 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
{{STRUCTURE_2hl7|  PDB=2hl7  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hl7 OCA], [https://pdbe.org/2hl7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hl7 RCSB], [https://www.ebi.ac.uk/pdbsum/2hl7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hl7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CCMH_PSEAE CCMH_PSEAE] Required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/2hl7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hl7 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CcmH (cytochromes c maturation protein H) is an essential component of the assembly line necessary for the maturation of c-type cytochromes in the periplasm of Gram-negative bacteria. The protein is a membrane-anchored thiol-oxidoreductase that has been hypothesized to be involved in the recognition and reduction of apocytochrome c, a prerequisite for covalent heme attachment. Here, we present the 1.7A crystal structure of the soluble periplasmic domain of CcmH from the opportunistic pathogen Pseudomonas aeruginosa (Pa-CcmH*). The protein contains a three-helix bundle, i.e. a fold that is different from that of all other thiol-oxidoreductases reported so far. The catalytic Cys residues of the conserved LRCXXC motif (Cys(25) and Cys(28)), located in a long loop connecting the first two helices, form a disulfide bond in the oxidized enzyme. We have determined the pK(a) values of these 2 Cys residues of Pa-CcmH* (both &gt;8) and propose a possible mechanistic role for a conserved Ser(36) and a water molecule in the active site. The interaction between Pa-CcmH* and Pa-apocyt c(551) (where cyt c(551) represents cytochrome c(551)) was characterized in vitro following the binding kinetics by stopped-flow using a Trp-containing fluorescent variant of Pa-CcmH* and a dansylated peptide, mimicking the apocytochrome c(551) heme binding motif. The kinetic results show that the protein has a moderate affinity to its apocyt substrate, consistent with the role of Pa-CcmH as an intermediate component of the assembly line for c-type cytochrome biogenesis.


===Crystal structure of the periplasmic domain of CcmH from Pseudomonas aeruginosa===
A strategic protein in cytochrome c maturation: three-dimensional structure of CcmH and binding to apocytochrome c.,Di Matteo A, Gianni S, Schinina ME, Giorgi A, Altieri F, Calosci N, Brunori M, Travaglini-Allocatelli C J Biol Chem. 2007 Sep 14;282(37):27012-9. Epub 2007 Jul 10. PMID:17623665<ref>PMID:17623665</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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(as it appears on PubMed at http://www.pubmed.gov), where 17623665 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_17623665}}
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</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2HL7 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HL7 OCA].
 
==Reference==
<ref group="xtra">PMID:17623665</ref><ref group="xtra">PMID:9914305</ref><ref group="xtra">PMID:16236729</ref><references group="xtra"/>
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Brunori, M.]]
[[Category: Brunori M]]
[[Category: Gianni, S.]]
[[Category: Di Matteo A]]
[[Category: Matteo, A Di.]]
[[Category: Gianni S]]
[[Category: Travaglini-Allocatelli, C.]]
[[Category: Travaglini-Allocatelli C]]
[[Category: Oxidoreductase]]
[[Category: Three-helices bundle]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 08:39:44 2009''
Retrieved from "https://proteopedia.org/2hl7"