2bye: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /><applet load="2bye" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bye" /> '''NMR SOLUTION STRUCTURE OF PHOSPHOLIPASE C EP... |
No edit summary |
||
| (14 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
== | ==NMR solution structure of phospholipase c epsilon RA 1 domain== | ||
Ras proteins signal to a number of distinct pathways by interacting with | <StructureSection load='2bye' size='340' side='right'caption='[[2bye]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2bye]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BYE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BYE FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bye OCA], [https://pdbe.org/2bye PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bye RCSB], [https://www.ebi.ac.uk/pdbsum/2bye PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bye ProSAT]</span></td></tr> | |||
</table> | |||
== Disease == | |||
[https://www.uniprot.org/uniprot/PLCE1_HUMAN PLCE1_HUMAN] Familial idiopathic steroid-resistant nephrotic syndrome with diffuse mesangial sclerosis;Familial idiopathic steroid-resistant nephrotic syndrome with focal segmental hyalinosis. The disease is caused by mutations affecting the gene represented in this entry. | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PLCE1_HUMAN PLCE1_HUMAN] The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation.<ref>PMID:11022047</ref> <ref>PMID:11395506</ref> <ref>PMID:11715024</ref> <ref>PMID:11877431</ref> <ref>PMID:12721365</ref> <ref>PMID:16537651</ref> <ref>PMID:17086182</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ras proteins signal to a number of distinct pathways by interacting with diverse effectors. Studies of ras/effector interactions have focused on three classes, Raf kinases, ral guanylnucleotide-exchange factors, and phosphatidylinositol-3-kinases. Here we describe ras interactions with another effector, the recently identified phospholipase C epsilon (PLCepsilon). We solved structures of PLCepsilon RA domains (RA1 and RA2) by NMR and the structure of the RA2/ras complex by X-ray crystallography. Although the similarity between ubiquitin-like folds of RA1 and RA2 proves that they are homologs, only RA2 can bind ras. Some of the features of the RA2/ras interface are unique to PLCepsilon, while the ability to make contacts with both switch I and II regions of ras is shared only with phosphatidylinositol-3-kinase. Studies of PLCepsilon regulation suggest that, in a cellular context, the RA2 domain, in a mode specific to PLCepsilon, has a role in membrane targeting with further regulatory impact on PLC activity. | |||
Structural and mechanistic insights into ras association domains of phospholipase C epsilon.,Bunney TD, Harris R, Gandarillas NL, Josephs MB, Roe SM, Sorli SC, Paterson HF, Rodrigues-Lima F, Esposito D, Ponting CP, Gierschik P, Pearl LH, Driscoll PC, Katan M Mol Cell. 2006 Feb 17;21(4):495-507. PMID:16483931<ref>PMID:16483931</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2bye" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Phospholipase C|Phospholipase C]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Bunney | [[Category: Bunney TD]] | ||
[[Category: Driscoll | [[Category: Driscoll PC]] | ||
[[Category: Esposito | [[Category: Esposito D]] | ||
[[Category: Gandarillas | [[Category: Gandarillas NL]] | ||
[[Category: Gieschik | [[Category: Gieschik P]] | ||
[[Category: Harris | [[Category: Harris R]] | ||
[[Category: Josephs | [[Category: Josephs MB]] | ||
[[Category: Katan | [[Category: Katan M]] | ||
[[Category: Paterson | [[Category: Paterson HF]] | ||
[[Category: Pearl | [[Category: Pearl LH]] | ||
[[Category: Rodrigues-Lima | [[Category: Rodrigues-Lima F]] | ||
[[Category: Roe | [[Category: Roe SM]] | ||
