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New page: left|200px<br /><applet load="2czw" size="450" color="white" frame="true" align="right" spinBox="true" caption="2czw, resolution 1.9Å" /> '''Crystal structure ana...
 
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[[Image:2czw.gif|left|200px]]<br /><applet load="2czw" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2czw, resolution 1.9&Aring;" />
'''Crystal structure analysis of protein component Ph1496p of P.horikoshii ribonuclease P'''<br />


==Overview==
==Crystal structure analysis of protein component Ph1496p of P.horikoshii ribonuclease P==
Ribonuclease P (RNase P) is a ribonucleoprotein complex involved in the, processing of the 5' leader sequence of precursor tRNA. We previously, found that the reconstituted particle (RP) composed of RNase P RNA and, four proteins (Ph1481p, Ph1601p, Ph1771p, and Ph1877p) in the, hyperthermophilic archaeon Pyrococcus horikoshii OT3 exhibited the RNase P, activity, but had a lower optimal temperature (around at 55 degrees C), as, compared with 70 degrees C of the authentic RNase P from P. horikoshii, [Kouzuma et al., Biochem. Biophys. Res. Commun. 306 (2003) 666-673]. In, the present study, we found that addition of a fifth protein Ph1496p, a, putative ribosomal protein L7Ae, to RP specifically elevated the optimum, temperature to about 70 degrees C comparable to that of the authentic, RNase P. Characterization using gel shift assay and chemical probing, localized Ph1496p binding sites on two stem-loop structures encompassing, nucleotides A116-G201 and G229-C276 in P. horikoshii RNase P RNA., Moreover, the crystal structure of Ph1496p was determined at 2.0 A, resolution by the molecular replacement method using ribosomal protein, L7Ae from Haloarcula marismortui as a search model. Ph1496p comprises five, alpha-helices and a four stranded beta-sheet. The beta-sheet is sandwiched, by three helices (alpha1, alpha4, and alpha5) at one side and two helices, (alpha2 and alpha3) at other side. The archaeal ribosomal protein L7Ae is, known to be a triple functional protein, serving as a protein component in, ribosome and ribonucleoprotein complexes, box C/D, and box H/ACA. Although, we have at present no direct evidence that Ph1496p is a real protein, component in the P. horikoshii RNase P, the present result may assign an, RNase P protein to L7Ae as a fourth function.
<StructureSection load='2czw' size='340' side='right'caption='[[2czw]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2czw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CZW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2czw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2czw OCA], [https://pdbe.org/2czw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2czw RCSB], [https://www.ebi.ac.uk/pdbsum/2czw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2czw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RL7A_PYRHO RL7A_PYRHO] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA, box C/D and box C'/D' sRNAs (By similarity). When added to reconstituted ribonuclease P (RNase P) it increases the optimum temperature to that of the partially purified enzyme and causes a 5-fold increase in apparent Vmax. Binds the RNase P catalytic RNA.<ref>PMID:16829535</ref> <ref>PMID:16574071</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cz/2czw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2czw ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2CZW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CZW OCA].
*[[Ribosomal protein L7/L12|Ribosomal protein L7/L12]]
 
== References ==
==Reference==
<references/>
A fifth protein subunit Ph1496p elevates the optimum temperature for the ribonuclease P activity from Pyrococcus horikoshii OT3., Fukuhara H, Kifusa M, Watanabe M, Terada A, Honda T, Numata T, Kakuta Y, Kimura M, Biochem Biophys Res Commun. 2006 May 12;343(3):956-64. Epub 2006 Mar 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16574071 16574071]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pyrococcus horikoshii]]
[[Category: Pyrococcus horikoshii]]
[[Category: Single protein]]
[[Category: Fukuhara H]]
[[Category: Fukuhara, H.]]
[[Category: Honda T]]
[[Category: Honda, T.]]
[[Category: Kakuta Y]]
[[Category: Kakuta, Y.]]
[[Category: Kifusa M]]
[[Category: Kifusa, M.]]
[[Category: Kimura M]]
[[Category: Kimura, M.]]
[[Category: Numata T]]
[[Category: Numata, T.]]
[[Category: Terada A]]
[[Category: Terada, A.]]
[[Category: Watanabe M]]
[[Category: Watanabe, M.]]
[[Category: ribonuclease p]]
[[Category: ribonucleoprotein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:22:17 2007''

Latest revision as of 13:45, 13 March 2024

Crystal structure analysis of protein component Ph1496p of P.horikoshii ribonuclease P

2czw, resolution 1.90Å

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