1h9h: Difference between revisions

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New page: left|200px<br /> <applet load="1h9h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h9h, resolution 1.5Å" /> '''COMPLEX OF EETI-II W...
 
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[[Image:1h9h.gif|left|200px]]<br />
<applet load="1h9h" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1h9h, resolution 1.5&Aring;" />
'''COMPLEX OF EETI-II WITH PORCINE TRYPSIN'''<br />


==About this Structure==
==COMPLEX OF EETI-II WITH PORCINE TRYPSIN==
1H9H is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Ecballium_elaterium Ecballium elaterium]] and [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H9H OCA]].
<StructureSection load='1h9h' size='340' side='right'caption='[[1h9h]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1h9h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecballium_elaterium Ecballium elaterium] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H9H FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9h OCA], [https://pdbe.org/1h9h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h9h RCSB], [https://www.ebi.ac.uk/pdbsum/1h9h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h9h ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TRYP_PIG TRYP_PIG]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h9/1h9h_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h9h ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of squash inhibitors and is one of the strongest inhibitors known for trypsin. The eight independent molecules of EETI-II in the crystal structure reported here provide a good opportunity to test the hypothesis that this small cystine-knot protein (knottin) is sufficiently rigid to be used as a molecular scaffold for protein-engineering purposes. To extend this test, the structures of two complexes of EETI-II with trypsin have also been determined, one carrying a four-amino-acid mutation of EETI-II. The remarkable similarity of these structures confirms the rigidity of the molecular framework and hence its suitability as a molecular scaffold.
 
Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold.,Kratzner R, Debreczeni JE, Pape T, Schneider TR, Wentzel A, Kolmar H, Sheldrick GM, Uson I Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1255-62. Epub 2005, Aug 16. PMID:16131759<ref>PMID:16131759</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1h9h" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Trypsin 3D structures|Trypsin 3D structures]]
*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Ecballium elaterium]]
[[Category: Ecballium elaterium]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
[[Category: Kolmar, H.]]
[[Category: Kolmar H]]
[[Category: Kraetzner, R.]]
[[Category: Kraetzner R]]
[[Category: Uson, I.]]
[[Category: Uson I]]
[[Category: Wentzel, A.]]
[[Category: Wentzel A]]
[[Category: CA]]
[[Category: cystine knot]]
[[Category: squash inhibitor]]
[[Category: trypsin]]
 
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Retrieved from "https://proteopedia.org/1h9h"