2evu: Difference between revisions

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New page: left|200px<br /><applet load="2evu" size="450" color="white" frame="true" align="right" spinBox="true" caption="2evu, resolution 2.3Å" /> '''Crystal structure of ...
 
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[[Image:2evu.gif|left|200px]]<br /><applet load="2evu" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2evu, resolution 2.3&Aring;" />
'''Crystal structure of aquaporin AqpM at 2.3A resolution'''<br />


==Overview==
==Crystal structure of aquaporin AqpM at 2.3A resolution==
To explore the structural basis of the unique selectivity spectrum and, conductance of the transmembrane channel protein AqpM from the archaeon, Methanothermobacter marburgensis, we determined the structure of AqpM to, 1.68-A resolution by x-ray crystallography. The structure establishes AqpM, as being in a unique subdivision between the two major subdivisions of, aquaporins, the water-selective aquaporins, and the, water-plus-glycerol-conducting aquaglyceroporins. In AqpM, isoleucine, replaces a key histidine residue found in the lumen of water channels, which becomes a glycine residue in aquaglyceroporins. As a result of this, and other side-chain substituents in the walls of the channel, the channel, is intermediate in size and exhibits differentially tuned electrostatics, when compared with the other subfamilies.
<StructureSection load='2evu' size='340' side='right'caption='[[2evu]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2evu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_marburgensis_str._Marburg Methanothermobacter marburgensis str. Marburg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EVU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2evu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2evu OCA], [https://pdbe.org/2evu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2evu RCSB], [https://www.ebi.ac.uk/pdbsum/2evu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2evu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AQPM_METTM AQPM_METTM] Channel that permits osmotically driven movement of water in both directions. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity. Exhibits also a transient but reproducible increase in the initial glycerol flux.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ev/2evu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2evu ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2EVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus] with BOG and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2EVU OCA].
*[[Aquaporin 3D structures|Aquaporin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structural basis for conductance by the archaeal aquaporin AqpM at 1.68 A., Lee JK, Kozono D, Remis J, Kitagawa Y, Agre P, Stroud RM, Proc Natl Acad Sci U S A. 2005 Dec 27;102(52):18932-7. Epub 2005 Dec 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16361443 16361443]
[[Category: Large Structures]]
[[Category: Methanothermobacter thermautotrophicus]]
[[Category: Methanothermobacter marburgensis str. Marburg]]
[[Category: Single protein]]
[[Category: Agre P]]
[[Category: Agre, P.]]
[[Category: Kitagawa Y]]
[[Category: Kitagawa, Y.]]
[[Category: Kozono D]]
[[Category: Kozono, D.]]
[[Category: Lee JK]]
[[Category: Lee, J.K.]]
[[Category: Remis J]]
[[Category: Remis, J.]]
[[Category: Stroud RM]]
[[Category: Stroud, R.M.]]
[[Category: BOG]]
[[Category: GOL]]
[[Category: alpha-helical]]
 
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