2exu: Difference between revisions

Latest revision as of 07:58, 30 October 2024

Crystal Structure of Saccharomyces cerevisiae transcription elongation factors Spt4-Spt5NGN domain

Structural highlights

2exu is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.23Å
Ligands:	EOH, MPD, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SPT4_YEAST The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. The complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene. Structural and functional component of the centromeric and heterochromatic loci linking chromatin structure with kinetochore function and gene silencing.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Malone EA, Fassler JS, Winston F. Molecular and genetic characterization of SPT4, a gene important for transcription initiation in Saccharomyces cerevisiae. Mol Gen Genet. 1993 Mar;237(3):449-59. PMID:8483459
↑ Hartzog GA, Wada T, Handa H, Winston F. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. Genes Dev. 1998 Feb 1;12(3):357-69. PMID:9450930
↑ Lindstrom DL, Squazzo SL, Muster N, Burckin TA, Wachter KC, Emigh CA, McCleery JA, Yates JR 3rd, Hartzog GA. Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol. 2003 Feb;23(4):1368-78. PMID:12556496
↑ Rondon AG, Garcia-Rubio M, Gonzalez-Barrera S, Aguilera A. Molecular evidence for a positive role of Spt4 in transcription elongation. EMBO J. 2003 Feb 3;22(3):612-20. PMID:12554661 doi:https://dx.doi.org/10.1093/emboj/cdg047
↑ Crotti LB, Basrai MA. Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae. EMBO J. 2004 Apr 21;23(8):1804-14. Epub 2004 Apr 1. PMID:15057281 doi:https://dx.doi.org/10.1038/sj.emboj.7600161
↑ Mason PB, Struhl K. Distinction and relationship between elongation rate and processivity of RNA polymerase II in vivo. Mol Cell. 2005 Mar 18;17(6):831-40. PMID:15780939 doi:https://dx.doi.org/S1097-2765(05)01116-0
↑ Xiao Y, Yang YH, Burckin TA, Shiue L, Hartzog GA, Segal MR. Analysis of a splice array experiment elucidates roles of chromatin elongation factor Spt4-5 in splicing. PLoS Comput Biol. 2005 Sep;1(4):e39. Epub 2005 Sep 16. PMID:16172632 doi:https://dx.doi.org/10.1371/journal.pcbi.0010039

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Malone EA, Fassler JS, Winston F. Molecular and genetic characterization of SPT4, a gene important for transcription initiation in Saccharomyces cerevisiae. Mol Gen Genet. 1993 Mar;237(3):449-59. PMID:8483459

[2] Hartzog GA, Wada T, Handa H, Winston F. Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. Genes Dev. 1998 Feb 1;12(3):357-69. PMID:9450930

[3] Lindstrom DL, Squazzo SL, Muster N, Burckin TA, Wachter KC, Emigh CA, McCleery JA, Yates JR 3rd, Hartzog GA. Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol. 2003 Feb;23(4):1368-78. PMID:12556496

[4] Rondon AG, Garcia-Rubio M, Gonzalez-Barrera S, Aguilera A. Molecular evidence for a positive role of Spt4 in transcription elongation. EMBO J. 2003 Feb 3;22(3):612-20. PMID:12554661 doi:https://dx.doi.org/10.1093/emboj/cdg047

[5] Crotti LB, Basrai MA. Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae. EMBO J. 2004 Apr 21;23(8):1804-14. Epub 2004 Apr 1. PMID:15057281 doi:https://dx.doi.org/10.1038/sj.emboj.7600161

[6] Mason PB, Struhl K. Distinction and relationship between elongation rate and processivity of RNA polymerase II in vivo. Mol Cell. 2005 Mar 18;17(6):831-40. PMID:15780939 doi:https://dx.doi.org/S1097-2765(05)01116-0

[7] Xiao Y, Yang YH, Burckin TA, Shiue L, Hartzog GA, Segal MR. Analysis of a splice array experiment elucidates roles of chromatin elongation factor Spt4-5 in splicing. PLoS Comput Biol. 2005 Sep;1(4):e39. Epub 2005 Sep 16. PMID:16172632 doi:https://dx.doi.org/10.1371/journal.pcbi.0010039

[1]

[2]

[3]

[4]

[5]

[6]

[7]

@@ Line 1: / Line 1: @@
-[[Image:2exu.jpg|left|200px]]<br /><applet load="2exu" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2exu, resolution 2.230&Aring;" />
-'''Crystal Structure of Saccharomyces cerevisiae transcription elongation factors Spt4-Spt5NGN domain'''<br />
-==About this Structure==
+==Crystal Structure of Saccharomyces cerevisiae transcription elongation factors Spt4-Spt5NGN domain==
-EXU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN, EOH and MPD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2EXU OCA].
+<StructureSection load='2exu' size='340' side='right'caption='[[2exu]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2exu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EXU FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2exu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2exu OCA], [https://pdbe.org/2exu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2exu RCSB], [https://www.ebi.ac.uk/pdbsum/2exu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2exu ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPT4_YEAST SPT4_YEAST] The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. The complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene. Structural and functional component of the centromeric and heterochromatic loci linking chromatin structure with kinetochore function and gene silencing.<ref>PMID:8483459</ref> <ref>PMID:9450930</ref> <ref>PMID:12556496</ref> <ref>PMID:12554661</ref> <ref>PMID:15057281</ref> <ref>PMID:15780939</ref> <ref>PMID:16172632</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/2exu_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2exu ConSurf].
+<div style="clear:both"></div>
+==See Also==
+*[[Elongation factor 3D structures|Elongation factor 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Single protein]]
+[[Category: Fang P]]
-[[Category: Fang, P.]]
+[[Category: Guo M]]
-[[Category: Guo, M.]]
+[[Category: Niu L]]
-[[Category: Niu, L.]]
+[[Category: Teng M]]
-[[Category: Teng, M.]]
+[[Category: Xu F]]
-[[Category: Xu, F.]]
-[[Category: EOH]]
-[[Category: MPD]]
-[[Category: ZN]]
-[[Category: helixs surrounding beta sheet]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:12:41 2007''

2exu: Difference between revisions

Latest revision as of 07:58, 30 October 2024

Crystal Structure of Saccharomyces cerevisiae transcription elongation factors Spt4-Spt5NGN domain

Structural highlights

Function

Evolutionary Conservation

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools