2z8s: Difference between revisions

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{{Seed}}
[[Image:2z8s.png|left|200px]]


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==Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid==
The line below this paragraph, containing "STRUCTURE_2z8s", creates the "Structure Box" on the page.
<StructureSection load='2z8s' size='340' side='right'caption='[[2z8s]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2z8s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z8S FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
{{STRUCTURE_2z8s|  PDB=2z8s  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8s OCA], [https://pdbe.org/2z8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z8s RCSB], [https://www.ebi.ac.uk/pdbsum/2z8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z8s ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/YESW_BACSU YESW_BACSU] Pectinolytic enzyme that degrades type I rhamnogalacturonan from plant cell walls and releases oligosaccharide products. Degrades rhamnogalacturonan, polygalacturonic acid, pectic acid and pectin.<ref>PMID:16682770</ref> <ref>PMID:17449691</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z8/2z8s_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z8s ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Rhamnogalacturonan (RG) lyase produced by plant pathogenic and saprophytic microbes plays an important role in degrading plant cell walls. An extracellular RG lyase YesW from saprophytic Bacillus subtilis is a member of polysaccharide lyase family 11 and cleaves glycoside bonds in polygalacturonan as well as RG type-I through a beta-elimination reaction. Crystal structures of YesW and its complex with galacturonan disaccharide, a reaction product analogue, were determined at 1.4 and 2.5 A resolutions with final R-factors of 16.4% and 16.6%, respectively. The enzyme is composed of an eight-bladed beta-propeller with a deep cleft in the center as a basic scaffold, and its structural fold has not been seen in polysaccharide lyases analyzed thus far. Structural analysis of the disaccharide-bound YesW and a site-directed mutagenesis study suggested that Arg-452 and Lys-535 stabilize the carboxyl group of the acidic polysaccharide molecule and Tyr-595 makes a stack interaction with the sugar pyranose ring. In addition to amino acid residues binding to the disaccharide, one calcium ion, which is coordinated by Asp-401, Glu-422, His-363, and His-399, may mediate the enzyme activity. This is, to our knowledge, the first report of a new structural category with a beta-propeller fold in polysaccharide lyases and provides structural insights into substrate binding by RG lyase.


===Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid===
A novel structural fold in polysaccharide lyases: Bacillus subtilis family 11 rhamnogalacturonan lyase YesW with an eight-bladed beta-propeller.,Ochiai A, Itoh T, Maruyama Y, Kawamata A, Mikami B, Hashimoto W, Murata K J Biol Chem. 2007 Dec 21;282(51):37134-45. Epub 2007 Oct 17. PMID:17947240<ref>PMID:17947240</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2z8s" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 17947240 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_17947240}}
__TOC__
 
</StructureSection>
==About this Structure==
2Z8S is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8S OCA].
 
==Reference==
<ref group="xtra">PMID:17947240</ref><references group="xtra"/>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Hashimoto, W.]]
[[Category: Large Structures]]
[[Category: Itoh, T.]]
[[Category: Hashimoto W]]
[[Category: Kawamata, A.]]
[[Category: Itoh T]]
[[Category: Maruyama, Y.]]
[[Category: Kawamata A]]
[[Category: Mikami, B.]]
[[Category: Maruyama Y]]
[[Category: Murata, K.]]
[[Category: Mikami B]]
[[Category: Ochiai, A.]]
[[Category: Murata K]]
[[Category: Beta-propeller]]
[[Category: Ochiai A]]
[[Category: Lyase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:36:08 2009''
Retrieved from "https://proteopedia.org/2z8s"