1ry7: Difference between revisions

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{{Seed}}
[[Image:1ry7.png|left|200px]]


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==Crystal Structure of the 3 Ig form of FGFR3c in complex with FGF1==
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<StructureSection load='1ry7' size='340' side='right'caption='[[1ry7]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1ry7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RY7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RY7 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ry7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ry7 OCA], [https://pdbe.org/1ry7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ry7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ry7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ry7 ProSAT]</span></td></tr>
{{STRUCTURE_1ry7|  PDB=1ry7  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/FGF1_HUMAN FGF1_HUMAN] Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.<ref>PMID:8663044</ref> <ref>PMID:16597617</ref> <ref>PMID:20145243</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ry/1ry7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ry7 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The prototypical fibroblast growth factor receptor (FGFR) extracellular domain consists of three Ig domains (D1-D3) of which the two membrane-proximal D2 and D3 domains and the interconnecting D2-D3 linker bear the determinants of ligand binding and specificity. In contrast, D1 and the D1-D2 linker are thought to play autoinhibitory roles in FGFR regulation. Here, we report the crystal structure of the three-Ig form of FGFR3c in complex with FGF1, an FGF that binds promiscuously to each of the seven principal FGFRs. In this structure, D1 and the D1-D2 linker are completely disordered, demonstrating that these regions are dispensable for FGF binding. Real-time binding experiments using surface plasmon resonance show that relative to two-Ig form, the three-Ig form of FGFR3c exhibits lower affinity for both FGF1 and heparin. Importantly, we demonstrate that this autoinhibition is mediated by intramolecular interactions of D1 and the D1-D2 linker with the minimal FGF and heparin-binding D2-D3 region. As in the FGF1-FGFR2c structure, but not the FGF1-FGFR1c structure, the alternatively spliced betaC'-betaE loop is ordered and interacts with FGF1 in the FGF1-FGFR3c structure. However, in contrast to the FGF1-FGFR2c structure in which the betaC'-betaE loop interacts with the beta-trefoil core region of FGF1, in the FGF1-FGFR3c structure, this loop interacts extensively with the N-terminal region of FGF1, underscoring the importance of the FGF1 N terminus in conferring receptor-binding affinity and promiscuity. Importantly, comparison of the three FGF1-FGFR structures shows that the flexibility of the betaC'-betaE loop is a major determinant of ligand-binding specificity and promiscuity.


===Crystal Structure of the 3 Ig form of FGFR3c in complex with FGF1===
Insights into the molecular basis for fibroblast growth factor receptor autoinhibition and ligand-binding promiscuity.,Olsen SK, Ibrahimi OA, Raucci A, Zhang F, Eliseenkova AV, Yayon A, Basilico C, Linhardt RJ, Schlessinger J, Mohammadi M Proc Natl Acad Sci U S A. 2004 Jan 27;101(4):935-40. Epub 2004 Jan 19. PMID:14732692<ref>PMID:14732692</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1ry7" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_14732692}}, adds the Publication Abstract to the page
*[[Fibroblast growth factor 3D structures|Fibroblast growth factor 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 14732692 is the PubMed ID number.
*[[Fibroblast growth factor receptor 3D receptor|Fibroblast growth factor receptor 3D receptor]]
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== References ==
{{ABSTRACT_PUBMED_14732692}}
<references/>
 
__TOC__
==About this Structure==
</StructureSection>
1RY7 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RY7 OCA].
 
==Reference==
<ref group="xtra">PMID:14732692</ref><references group="xtra"/>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Basilico, C.]]
[[Category: Large Structures]]
[[Category: Eliseenkova, A V.]]
[[Category: Basilico C]]
[[Category: Ibrahimi, O A.]]
[[Category: Eliseenkova AV]]
[[Category: Linhardt, R J.]]
[[Category: Ibrahimi OA]]
[[Category: Mohammadi, M.]]
[[Category: Linhardt RJ]]
[[Category: Olsen, S K.]]
[[Category: Mohammadi M]]
[[Category: Raucci, A.]]
[[Category: Olsen SK]]
[[Category: Schlessinger, J.]]
[[Category: Raucci A]]
[[Category: Yayon, A.]]
[[Category: Schlessinger J]]
[[Category: Zhang, F.]]
[[Category: Yayon A]]
[[Category: Beta trefoil]]
[[Category: Zhang F]]
[[Category: Fgf-fgfr complex]]
[[Category: Ig domain]]
 
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