1u7v: Difference between revisions

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{{Seed}}
[[Image:1u7v.png|left|200px]]


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==Crystal Structure of the phosphorylated Smad2/Smad4 heterotrimeric complex==
The line below this paragraph, containing "STRUCTURE_1u7v", creates the "Structure Box" on the page.
<StructureSection load='1u7v' size='340' side='right'caption='[[1u7v]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1u7v]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U7V FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
{{STRUCTURE_1u7v|  PDB=1u7v  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u7v OCA], [https://pdbe.org/1u7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u7v RCSB], [https://www.ebi.ac.uk/pdbsum/1u7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u7v ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SMAD2_HUMAN SMAD2_HUMAN] Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.<ref>PMID:9892009</ref> <ref>PMID:16751101</ref> <ref>PMID:17327236</ref> <ref>PMID:16862174</ref> <ref>PMID:19289081</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u7/1u7v_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u7v ConSurf].
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== Publication Abstract from PubMed ==
The formation of protein complexes between phosphorylated R-Smads and Smad4 is a central event in the TGF-beta signaling pathway. We have determined the crystal structure of two R-Smad/Smad4 complexes, Smad3/Smad4 to 2.5 angstroms, and Smad2/Smad4 to 2.7 angstroms. Both complexes are heterotrimers, comprising two phosphorylated R-Smad subunits and one Smad4 subunit, a finding that was corroborated by isothermal titration calorimetry and mutational studies. Preferential formation of the R-Smad/Smad4 heterotrimer over the R-Smad homotrimer is largely enthalpy driven, contributed by the unique presence of strong electrostatic interactions within the heterotrimeric interfaces. The study supports a common mechanism of Smad protein assembly in TGF-beta superfamily signaling.


===Crystal Structure of the phosphorylated Smad2/Smad4 heterotrimeric complex===
Structural basis of heteromeric smad protein assembly in TGF-beta signaling.,Chacko BM, Qin BY, Tiwari A, Shi G, Lam S, Hayward LJ, De Caestecker M, Lin K Mol Cell. 2004 Sep 10;15(5):813-23. PMID:15350224<ref>PMID:15350224</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 1u7v" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 15350224 is the PubMed ID number.
== References ==
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<references/>
{{ABSTRACT_PUBMED_15350224}}
__TOC__
 
</StructureSection>
==About this Structure==
1U7V is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7V OCA].
 
==Reference==
<ref group="xtra">PMID:15350224</ref><references group="xtra"/>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Caestecker, M de.]]
[[Category: Large Structures]]
[[Category: Chacko, B M.]]
[[Category: Chacko BM]]
[[Category: Hayward, L J.]]
[[Category: Hayward LJ]]
[[Category: Lam, S.]]
[[Category: Lam S]]
[[Category: Lin, K.]]
[[Category: Lin K]]
[[Category: Qin, B Y.]]
[[Category: Qin BY]]
[[Category: Shi, G.]]
[[Category: Shi G]]
[[Category: Tiwari, A.]]
[[Category: Tiwari A]]
[[Category: Phosphorylation]]
[[Category: De Caestecker M]]
[[Category: Protein complex]]
[[Category: Signal transduction]]
[[Category: Smad]]
[[Category: Tgf-beta]]
 
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Retrieved from "https://proteopedia.org/1u7v"