2fqm: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px<br /><applet load="2fqm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fqm, resolution 2.3Å" /> '''Crystal structure of ...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:2fqm.gif|left|200px]]<br /><applet load="2fqm" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2fqm, resolution 2.3&Aring;" />
'''Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus'''<br />


==Overview==
==Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus==
In the replication cycle of nonsegmented negative-strand RNA viruses, the, viral RNA-dependent RNA polymerase (L) recognizes a nucleoprotein, (N)-enwrapped RNA template during the RNA polymerase reaction. The viral, phosphoprotein (P) is a polymerase cofactor essential for this, recognition. We report here the 2.3-angstroms-resolution crystal structure, of the central domain (residues 107 to 177) of P from vesicular stomatitis, virus. The fold of this domain consists of a beta hairpin, an alpha helix, and another beta hairpin. The alpha helix provides the stabilizing force, for forming a homodimer, while the two beta hairpins add additional, stabilization by forming a four-stranded beta sheet through domain, swapping between two molecules. This central dimer positions the N- and, C-terminal domains of P to interact with the N and L proteins, allowing, the L protein to specifically recognize the nucleocapsid-RNA template and, to progress along the template while concomitantly assembling N with, nascent RNA. The interdimer interactions observed in the, noncrystallographic packing may offer insight into the mechanism of the, RNA polymerase processive reaction along the viral nucleocapsid-RNA, template.
<StructureSection load='2fqm' size='340' side='right'caption='[[2fqm]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2fqm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus Vesicular stomatitis Indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FQM FirstGlance]. <br>
2FQM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FQM OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fqm OCA], [https://pdbe.org/2fqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fqm RCSB], [https://www.ebi.ac.uk/pdbsum/2fqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fqm ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus., Ding H, Green TJ, Lu S, Luo M, J Virol. 2006 Mar;80(6):2808-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16501089 16501089]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/PHOSP_VSIVM PHOSP_VSIVM] Essential component of the RNA polymerase transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template. May act as a chaperone for newly synthesized free N protein, so-called N(0). Plays a role in virion assembly (By similarity).
[[Category: Vesicular stomatitis indiana virus]]
== Evolutionary Conservation ==
[[Category: Ding, H.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Green, T.J.]]
Check<jmol>
[[Category: Lu, S.]]
  <jmolCheckbox>
[[Category: Luo, M.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fq/2fqm_consurf.spt"</scriptWhenChecked>
[[Category: cofactor]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: negative strand rna virus]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: polymerase]]
  </jmolCheckbox>
[[Category: replication]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fqm ConSurf].
 
<div style="clear:both"></div>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:42:34 2007''
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vesicular stomatitis Indiana virus]]
[[Category: Ding H]]
[[Category: Green TJ]]
[[Category: Lu S]]
[[Category: Luo M]]

Latest revision as of 09:24, 14 February 2024

Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus

2fqm, resolution 2.30Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2fqm&oldid=4058514"