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New page: left|200px<br /><applet load="2g5h" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g5h, resolution 2.5Å" /> '''Structure of tRNA-Dep...
 
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[[Image:2g5h.gif|left|200px]]<br /><applet load="2g5h" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2g5h, resolution 2.5&Aring;" />
'''Structure of tRNA-Dependent Amidotransferase GatCAB'''<br />


==Overview==
==Structure of tRNA-Dependent Amidotransferase GatCAB==
The formation of glutaminyl transfer RNA (Gln-tRNA(Gln)) differs among the, three domains of life. Most bacteria employ an indirect pathway to produce, Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB), that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of, crystal structures of intact GatCAB from Staphylococcus aureus in the apo, form and in the complexes with glutamine, asparagine, Mn2+, and adenosine, triphosphate analog. Two identified catalytic centers for the glutaminase, and transamidase reactions are markedly distant but connected by a, hydrophilic ammonia channel 30 A in length. Further, we show that the, first U-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve, as identity elements essential for discrimination by GatCAB and propose a, complete model for the overall concerted reactions to synthesize, Gln-tRNA(Gln).
<StructureSection load='2g5h' size='340' side='right'caption='[[2g5h]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2g5h]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G5H FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5h OCA], [https://pdbe.org/2g5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g5h RCSB], [https://www.ebi.ac.uk/pdbsum/2g5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g5h ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GATA_STAAM GATA_STAAM] Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/2g5h_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g5h ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2G5H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2G5H OCA].
*[[Glutamyl-tRNA(Gln) amidotransferase|Glutamyl-tRNA(Gln) amidotransferase]]
 
__TOC__
==Reference==
</StructureSection>
Ammonia channel couples glutaminase with transamidase reactions in GatCAB., Nakamura A, Yao M, Chimnaronk S, Sakai N, Tanaka I, Science. 2006 Jun 30;312(5782):1954-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16809541 16809541]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
[[Category: Nakamura, A.]]
[[Category: Nakamura A]]
[[Category: Tanaka, I.]]
[[Category: Tanaka I]]
[[Category: Yao, M.]]
[[Category: Yao M]]
[[Category: MG]]
[[Category: multi protein complex]]
 
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