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New page: left|200px<br /><applet load="2gek" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gek, resolution 2.400Å" /> '''Crystal Structure o...
 
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[[Image:2gek.jpg|left|200px]]<br /><applet load="2gek" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2gek, resolution 2.400&Aring;" />
'''Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP'''<br />


==Overview==
==Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP==
Phosphatidylinositol mannosyltransferase (PimA) is an essential enzyme for, mycobacterial growth that catalyses the first mannosylation step in, phosphatidyl-myo-inositol mannoside (PIM) biosynthesis. The enzyme belongs, to the large GT4 family of glycosyltransferases, for which no structure is, currently available. Recombinant purified PimA from Mycobacterium, smegmatis has been crystallized in the presence of GDP and myo-inositol., The crystals belong to space group P2(1)2(1)2(1), with unit-cell, parameters a = 37.2, b = 72.4, c = 138.2 A, and diffract to 2.4 A, resolution.
<StructureSection load='2gek' size='340' side='right'caption='[[2gek]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2gek]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GEK FirstGlance]. <br>
2GEK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis] with GDP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GEK OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gek OCA], [https://pdbe.org/2gek PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gek RCSB], [https://www.ebi.ac.uk/pdbsum/2gek PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gek ProSAT]</span></td></tr>
Crystallization and preliminary crystallographic analysis of PimA, an essential mannosyltransferase from Mycobacterium smegmatis., Guerin ME, Buschiazzo A, Kordulakova J, Jackson M, Alzari PM, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 May 1;61(Pt, 5):518-20. Epub 2005 Apr 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511084 16511084]
</table>
[[Category: Mycobacterium smegmatis]]
== Function ==
[[Category: Protein complex]]
[https://www.uniprot.org/uniprot/PIMA_MYCS2 PIMA_MYCS2] Catalyzes the addition of a mannose residue from GDP-D-mannose to the position 2 of a phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Contrary to PimB, the mannosyltransferase PimA is unable to transfer a mannose residue to the position 6 of the phosphatidyl-myo-inositola of PIM1.<ref>PMID:12068013</ref> <ref>PMID:19638342</ref>
[[Category: Alzari, P.M.]]
== Evolutionary Conservation ==
[[Category: Buschiazzo, A.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Guerin, M.E.]]
Check<jmol>
[[Category: Jackson, M.]]
  <jmolCheckbox>
[[Category: Kordulakova, J.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/2gek_consurf.spt"</scriptWhenChecked>
[[Category: GDP]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: binary complex]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: gt4 glycosyltransferase]]
  </jmolCheckbox>
[[Category: mannosyltransferase]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gek ConSurf].
[[Category: rossmann fold]]
<div style="clear:both"></div>
 
== References ==
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:09:25 2007''
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycolicibacterium smegmatis MC2 155]]
[[Category: Alzari PM]]
[[Category: Buschiazzo A]]
[[Category: Guerin ME]]
[[Category: Jackson M]]
[[Category: Kordulakova J]]

Latest revision as of 09:27, 14 February 2024

Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP

2gek, resolution 2.40Å

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