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New page: left|200px<br /><applet load="2gum" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gum, resolution 2.10Å" /> '''Crystal structure of...
 
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[[Image:2gum.gif|left|200px]]<br /><applet load="2gum" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2gum, resolution 2.10&Aring;" />
'''Crystal structure of the extracellular domain of glycoprotein B from Herpes Simplex Virus type I'''<br />


==Overview==
==Crystal structure of the extracellular domain of glycoprotein B from Herpes Simplex Virus type I==
Glycoprotein B (gB) is the most conserved component of the complex, cell-entry machinery of herpes viruses. A crystal structure of the gB, ectodomain from herpes simplex virus type 1 reveals a multidomain trimer, with unexpected homology to glycoprotein G from vesicular stomatitis virus, (VSV G). An alpha-helical coiled-coil core relates gB to class I viral, membrane fusion glycoproteins; two extended beta hairpins with hydrophobic, tips, homologous to fusion peptides in VSV G, relate gB to class II fusion, proteins. Members of both classes accomplish fusion through a large-scale, conformational change, triggered by a signal from a receptor-binding, component. The domain connectivity within a gB monomer would permit such a, rearrangement, including long-range translocations linked to viral and, cellular membranes.
<StructureSection load='2gum' size='340' side='right'caption='[[2gum]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2gum]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_alphaherpesvirus_1_strain_KOS Human alphaherpesvirus 1 strain KOS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GUM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gum OCA], [https://pdbe.org/2gum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gum RCSB], [https://www.ebi.ac.uk/pdbsum/2gum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gum ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GB_HHV1K GB_HHV1K] Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan sulfate moities of the host cell surface proteoglycans. Involved in fusion of viral and cellular membranes leading to virus entry into the host cell. Following initial binding of gD to one of its receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion egress. Also plays a role, together with gK, in virus-induced cell-to-cell fusion (syncytia formation).<ref>PMID:17299053</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/2gum_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gum ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glycoprotein B (gB) is the most conserved component of the complex cell-entry machinery of herpes viruses. A crystal structure of the gB ectodomain from herpes simplex virus type 1 reveals a multidomain trimer with unexpected homology to glycoprotein G from vesicular stomatitis virus (VSV G). An alpha-helical coiled-coil core relates gB to class I viral membrane fusion glycoproteins; two extended beta hairpins with hydrophobic tips, homologous to fusion peptides in VSV G, relate gB to class II fusion proteins. Members of both classes accomplish fusion through a large-scale conformational change, triggered by a signal from a receptor-binding component. The domain connectivity within a gB monomer would permit such a rearrangement, including long-range translocations linked to viral and cellular membranes.


==About this Structure==
Crystal structure of glycoprotein B from herpes simplex virus 1.,Heldwein EE, Lou H, Bender FC, Cohen GH, Eisenberg RJ, Harrison SC Science. 2006 Jul 14;313(5784):217-20. PMID:16840698<ref>PMID:16840698</ref>
2GUM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4] with NA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GUM OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of glycoprotein B from herpes simplex virus 1., Heldwein EE, Lou H, Bender FC, Cohen GH, Eisenberg RJ, Harrison SC, Science. 2006 Jul 14;313(5784):217-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16840698 16840698]
</div>
[[Category: Human herpesvirus 4]]
<div class="pdbe-citations 2gum" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
[[Category: Heldwein, E.E.]]
[[Category: NA]]
[[Category: envelope glycoprotein]]
[[Category: membrane fusion]]
[[Category: virus]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:24:02 2007''
==See Also==
*[[Glycoproteins B and D|Glycoproteins B and D]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human alphaherpesvirus 1 strain KOS]]
[[Category: Large Structures]]
[[Category: Heldwein EE]]
Retrieved from "https://proteopedia.org/2gum"