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New page: left|200px<br /><applet load="2hap" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hap, resolution 2.5Å" /> '''STRUCTURE OF A HAP1-1...
 
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[[Image:2hap.gif|left|200px]]<br /><applet load="2hap" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2hap, resolution 2.5&Aring;" />
'''STRUCTURE OF A HAP1-18/DNA COMPLEX REVEALS THAT PROTEIN/DNA INTERACTIONS CAN HAVE DIRECT ALLOSTERIC EFFECTS ON TRANSCRIPTIONAL ACTIVATION'''<br />


==Overview==
==STRUCTURE OF A HAP1-18/DNA COMPLEX REVEALS THAT PROTEIN/DNA INTERACTIONS CAN HAVE DIRECT ALLOSTERIC EFFECTS ON TRANSCRIPTIONAL ACTIVATION==
HAP1 is a yeast transcriptional activator that binds with equal affinity, to the dissimilar upstream activation sequences UAS1 and UAS(CYC7), but, activates transcription differentially when bound to each site. HAP1-18, harbors an amino acid change in the DNA binding domain. While binding UAS1, poorly, HAP1-18 binds UAS(CYC7) with wild-type properties and activates, transcription at elevated levels relative to HAP1. We have determined the, structure of HAP1-18-UAS(CYC7) and have compared it to HAP1-UAS(CYC7)., Unexpectedly, the single amino acid substitution in HAP1-18 nucleates a, significantly altered hydrogen bond interface between the protein and DNA, resulting in DNA conformational changes and an ordering of one N-terminal, arm of the protein dimer along the DNA minor groove. These observations, together with a large subset of transcriptionally defective mutations in, the HAP1 DNA-binding domain that map to the HAP1-DNA interface, suggest, that protein-DNA interactions may have direct allosteric effects on, transcriptional activation.
<StructureSection load='2hap' size='340' side='right'caption='[[2hap]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2hap]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HAP FirstGlance]. <br>
2HAP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HAP OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hap OCA], [https://pdbe.org/2hap PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hap RCSB], [https://www.ebi.ac.uk/pdbsum/2hap PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hap ProSAT]</span></td></tr>
Structure of HAP1-18-DNA implicates direct allosteric effect of protein-DNA interactions on transcriptional activation., King DA, Zhang L, Guarente L, Marmorstein R, Nat Struct Biol. 1999 Jan;6(1):22-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9886287 9886287]
</table>
== Function ==
[https://www.uniprot.org/uniprot/HAP1_YEASX HAP1_YEASX] Regulation of oxygen dependent gene expression. It modulates the expression of Iso-1 (CYP1) and Iso-2 (CYP3) cytochrome c. In response to heme, promotes transcription of genes encoding functions required for respiration, controlling oxidative damage and repression of anaerobic genes. Binds to the sequence 5'-CGGNNNTNNCGG-3'. Is non-functional in terms of iso-1 cytochrome c expression in strain S288c and its derivatives.<ref>PMID:10541856</ref> <ref>PMID:11689685</ref> <ref>PMID:2851658</ref> <ref>PMID:9027731</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ha/2hap_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hap ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Guarente L]]
[[Category: Guarente, L.]]
[[Category: King DA]]
[[Category: King, D.A.]]
[[Category: Marmorstein R]]
[[Category: Marmorstein, R.]]
[[Category: Zhang L]]
[[Category: Zhang, L.]]
[[Category: ZN]]
[[Category: asymmetry]]
[[Category: complex transcription factor/dna]]
[[Category: hyperactive mutant]]
[[Category: transcriptional activation]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:38:50 2007''
Retrieved from "https://proteopedia.org/2hap"