1epu: Difference between revisions

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{{Seed}}
[[Image:1epu.png|left|200px]]


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==X-RAY crystal structure of neuronal SEC1 from squid==
The line below this paragraph, containing "STRUCTURE_1epu", creates the "Structure Box" on the page.
<StructureSection load='1epu' size='340' side='right'caption='[[1epu]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1epu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Doryteuthis_pealeii Doryteuthis pealeii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EPU FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
{{STRUCTURE_1epu|  PDB=1epu  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1epu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1epu OCA], [https://pdbe.org/1epu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1epu RCSB], [https://www.ebi.ac.uk/pdbsum/1epu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1epu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O62547_DORPE O62547_DORPE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ep/1epu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1epu ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: Sec1-like molecules have been implicated in a variety of eukaryotic vesicle transport processes including neurotransmitter release by exocytosis. They regulate vesicle transport by binding to a t-SNARE from the syntaxin family. This process is thought to prevent SNARE complex formation, a protein complex required for membrane fusion. Whereas Sec1 molecules are essential for neurotransmitter release and other secretory events, their interaction with syntaxin molecules seems to represent a negative regulatory step in secretion. RESULTS: Here we report the X-ray crystal structure of a neuronal Sec1 homologue from squid, s-Sec1, at 2.4 A resolution. Neuronal s-Sec1 is a modular protein that folds into a V-shaped three-domain assembly. Peptide and mutagenesis studies are discussed with respect to the mechanism of Sec1 regulation. Comparison of the structure of squid s-Sec1 with the previously determined structure of rat neuronal Sec1 (n-Sec1) bound to syntaxin-1a indicates conformational rearrangements in domain III induced by syntaxin binding. CONCLUSIONS: The crystal structure of s-Sec1 provides the molecular scaffold for a number of molecular interactions that have been reported to affect Sec1 function. The structural differences observed between s-Sec1 and the structure of a rat n-Sec1-syntaxin-1a complex suggest that local conformational changes are sufficient to release syntaxin-1a from neuronal Sec1, an active process that is thought to involve additional effector molecule(s).


===X-RAY CRYSTAL STRUCTURE OF NEURONAL SEC1 FROM SQUID===
The X-ray crystal structure of neuronal Sec1 from squid sheds new light on the role of this protein in exocytosis.,Bracher A, Perrakis A, Dresbach T, Betz H, Weissenhorn W Structure. 2000 Jul 15;8(7):685-94. PMID:10903948<ref>PMID:10903948</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
The line below this paragraph, {{ABSTRACT_PUBMED_10903948}}, adds the Publication Abstract to the page
<div class="pdbe-citations 1epu" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 10903948 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_10903948}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Doryteuthis pealeii]]
1EPU is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Loligo_pealei Loligo pealei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EPU OCA].
[[Category: Large Structures]]
 
[[Category: Betz H]]
==Reference==
[[Category: Bracher A]]
<ref group="xtra">PMID:10903948</ref><references group="xtra"/>
[[Category: Dresbach T]]
[[Category: Loligo pealei]]
[[Category: Perrakis A]]
[[Category: Betz, H.]]
[[Category: Weissenhorn W]]
[[Category: Bracher, A.]]
[[Category: Dresbach, T.]]
[[Category: Perrakis, A.]]
[[Category: Weissenhorn, W.]]
[[Category: Helical bundle]]
[[Category: Left-hand turn connection]]
[[Category: Parallel beta-sheet]]
 
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Retrieved from "https://proteopedia.org/1epu"