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New page: left|200px<br /><applet load="2hes" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hes, resolution 1.700Å" /> '''Cytosolic Iron-sulp...
 
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[[Image:2hes.jpg|left|200px]]<br /><applet load="2hes" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2hes, resolution 1.700&Aring;" />
'''Cytosolic Iron-sulphur Assembly Protein- 1'''<br />


==Overview==
==Cytosolic Iron-sulphur Assembly Protein- 1==
The WD40-repeat protein Cia1 is an essential, conserved member of the, cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery in, eukaryotes. Here, we report the crystal structure of Saccharomyces, cerevisiae Cia1 to 1.7 A resolution. The structure folds into a beta, propeller with seven blades pseudo symmetrically arranged around a central, axis. Structure-based sequence alignment of Cia1 proteins shows that the, WD40 propeller core elements are highly conserved. Site-directed, mutagenesis of amino acid residues in loop regions with high solvent, accessibility identified that the conserved top surface residue R127, performs a critical function: the R127 mutant cells grew slowly and were, impaired in cytosolic Fe/S protein assembly. Human Ciao1, which reportedly, interacts with the Wilms' tumor suppressor, WT1, is structurally similar, to yeast Cia1. We show that Ciao1 can functionally replace Cia1 and, support cytosolic Fe/S protein biogenesis. Hence, our structural and, biochemical studies indicate the conservation of Cia1 function in, eukaryotes.
<StructureSection load='2hes' size='340' side='right'caption='[[2hes]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2hes]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HES OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HES FirstGlance]. <br>
2HES is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HES OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hes OCA], [https://pdbe.org/2hes PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hes RCSB], [https://www.ebi.ac.uk/pdbsum/2hes PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hes ProSAT]</span></td></tr>
Structure of the Yeast WD40 Domain Protein Cia1, a Component Acting Late in Iron-Sulfur Protein Biogenesis., Srinivasan V, Netz DJ, Webert H, Mascarenhas J, Pierik AJ, Michel H, Lill R, Structure. 2007 Oct 16;15(10):1246-1257. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17937914 17937914]
</table>
== Function ==
[https://www.uniprot.org/uniprot/CIAO1_YEAST CIAO1_YEAST] Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.<ref>PMID:16314508</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/he/2hes_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hes ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Daili JAN]]
[[Category: Daili, J.A.N.]]
[[Category: Lill R]]
[[Category: Lill, R.]]
[[Category: Michel H]]
[[Category: Michel, H.]]
[[Category: Pierik AJ]]
[[Category: Pierik, A.J.]]
[[Category: Srinivasan V]]
[[Category: Srinivasan, V.]]
[[Category: CA]]
[[Category: beta-propeller]]
[[Category: biosynthetic protein]]
[[Category: wd40 repeat]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:42:17 2007''

Latest revision as of 09:32, 14 February 2024

Cytosolic Iron-sulphur Assembly Protein- 1

2hes, resolution 1.70Å

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