2keq: Difference between revisions
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New page: '''Unreleased structure''' The entry 2keq is ON HOLD Authors: Oeemig, J.S., Aranko, A., Djupsj, J.B., Iwai, H. Description: Solution structure of DnaE intein from Nostoc punctiforme '... |
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==Solution structure of DnaE intein from Nostoc punctiforme== | |||
<StructureSection load='2keq' size='340' side='right'caption='[[2keq]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2keq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_punctiforme_PCC_73102 Nostoc punctiforme PCC 73102]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KEQ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2keq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2keq OCA], [https://pdbe.org/2keq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2keq RCSB], [https://www.ebi.ac.uk/pdbsum/2keq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2keq ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B2J066_NOSP7 B2J066_NOSP7] [https://www.uniprot.org/uniprot/B2J821_NOSP7 B2J821_NOSP7] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ke/2keq_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2keq ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Naturally split DnaE intein from Nostoc punctiforme (Npu) has robust protein trans-splicing activity and high tolerance of sequence variations at the splicing junctions. We determined the solution structure of a single chain variant of NpuDnaE intein by NMR spectroscopy. Based on the NMR structure and the backbone dynamics of the single chain NpuDnaE intein, we designed a functional split variant of the NpuDnaE intein having a short C-terminal half (C-intein) composed of six residues. In vivo and in vitro protein ligation of model proteins by the newly designed split intein were demonstrated. | |||
Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification.,Oeemig JS, Aranko AS, Djupsjobacka J, Heinamaki K, Iwai H FEBS Lett. 2009 May 6;583(9):1451-6. Epub 2009 Apr 1. PMID:19344715<ref>PMID:19344715</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2keq" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[DNA polymerase 3D structures|DNA polymerase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Nostoc punctiforme PCC 73102]] | |||
[[Category: Aranko AS]] | |||
[[Category: Djupsj JB]] | |||
[[Category: Iwai H]] | |||
[[Category: Oeemig JS]] | |||
