3g37: Difference between revisions

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New page: '''Unreleased structure''' The entry 3g37 is ON HOLD Authors: Murakami, K., Narita, A., Yasunaga, T., Noguchi, T.Q.P., Uyeda, T.Q.P., Wakabayashi, T. Description: Cryo-EM structure of ...
 
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'''Unreleased structure'''


The entry 3g37 is ON HOLD
==Cryo-EM structure of actin filament in the presence of phosphate==
<SX load='3g37' size='340' side='right' viewer='molstar' caption='[[3g37]], [[Resolution|resolution]] 6.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3g37]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3G37 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3G37 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3g37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3g37 OCA], [https://pdbe.org/3g37 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3g37 RCSB], [https://www.ebi.ac.uk/pdbsum/3g37 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3g37 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Assembled actin filaments support cellular signaling, intracellular trafficking, and cytokinesis. ATP hydrolysis triggered by actin assembly provides the structural cues for filament turnover in vivo. Here, we present the cryo-electron microscopic (cryo-EM) structure of filamentous actin (F-actin) in the presence of phosphate, with the visualization of some alpha-helical backbones and large side chains. A complete atomic model based on the EM map identified intermolecular interactions mediated by bound magnesium and phosphate ions. Comparison of the F-actin model with G-actin monomer crystal structures reveals a critical role for bending of the conserved proline-rich loop in triggering phosphate release following ATP hydrolysis. Crystal structures of G-actin show that mutations in this loop trap the catalytic site in two intermediate states of the ATPase cycle. The combined structural information allows us to propose a detailed molecular mechanism for the biochemical events, including actin polymerization and ATPase activation, critical for actin filament dynamics.


Authors: Murakami, K., Narita, A., Yasunaga, T., Noguchi, T.Q.P., Uyeda, T.Q.P., Wakabayashi, T.
Structural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release.,Murakami K, Yasunaga T, Noguchi TQ, Gomibuchi Y, Ngo KX, Uyeda TQ, Wakabayashi T Cell. 2010 Oct 15;143(2):275-87. PMID:20946985<ref>PMID:20946985</ref>


Description: Cryo-EM structure of actin filament
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3g37" style="background-color:#fffaf0;"></div>


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 08:54:32 2009''
==See Also==
*[[Actin 3D structures|Actin 3D structures]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Murakami K]]
[[Category: Noguchi TQ]]
[[Category: Uyeda TQ]]
[[Category: Wakabayshi T]]
[[Category: Yasunaga T]]
Retrieved from "https://proteopedia.org/3g37"