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New page: left|200px<br /><applet load="2iew" size="450" color="white" frame="true" align="right" spinBox="true" caption="2iew, resolution 2.00Å" /> '''Crystal structure of...
 
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[[Image:2iew.jpg|left|200px]]<br /><applet load="2iew" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2iew, resolution 2.00&Aring;" />
'''Crystal structure of Inositol Phosphate Multikinase Ipk2 from S. cerevisiae'''<br />


==Overview==
==Crystal structure of Inositol Phosphate Multikinase Ipk2 from S. cerevisiae==
Inositol polyphosphates perform essential functions as second messengers, in eukaryotic cells, and their cellular levels are regulated by inositol, phosphate kinases. Most of these enzymes belong to the inositol phosphate, kinase superfamily, which consists of three subgroups, inositol 3-kinases, inositol phosphate multikinases, and inositol hexakisphosphate kinases., Family members share several strictly conserved signature motifs and are, expected to have the same backbone fold, despite very limited overall, amino acid sequence identity. Sequence differences are expected to play, important roles in defining the different substrate selectivity of these, enzymes. To investigate the structural basis for substrate specificity, we, have determined the crystal structure of the yeast inositol phosphate, multikinase Ipk2 in the apoform and in a complex with ADP and Mn(2+) at up, to 2.0A resolution. The overall structure of Ipk2 is related to inositol, trisphosphate 3-kinase. The ATP binding site is similar in both enzymes;, however, the inositol binding domain is significantly smaller in Ipk2., Replacement of critical side chains in the inositolbinding site suggests, how modification of substrate recognition motifs determines enzymatic, substrate preference and catalysis.
<StructureSection load='2iew' size='340' side='right'caption='[[2iew]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2iew]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IEW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IEW FirstGlance]. <br>
2IEW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Inositol-polyphosphate_multikinase Inositol-polyphosphate multikinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.151 2.7.1.151] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IEW OCA].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iew OCA], [https://pdbe.org/2iew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iew RCSB], [https://www.ebi.ac.uk/pdbsum/2iew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iew ProSAT]</span></td></tr>
Crystal structure of inositol phosphate multikinase 2 and implications for substrate specificity., Holmes W, Jogl G, J Biol Chem. 2006 Dec 8;281(49):38109-16. Epub 2006 Oct 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17050532 17050532]
</table>
[[Category: Inositol-polyphosphate multikinase]]
== Function ==
[https://www.uniprot.org/uniprot/IPMK_YEAST IPMK_YEAST] Has kinase activity and phosphorylates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4). Has low kinase activity towards InsP6. With ARG80, ARG81 and MCM1, coordinates the expression of arginine anabolic and catabolic genes in response to arginine. Recruites ARG80 and MCM21 to stabilize them.<ref>PMID:10574768</ref> <ref>PMID:10632874</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/2iew_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iew ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Holmes W]]
[[Category: Holmes, W.]]
[[Category: Jogl G]]
[[Category: Jogl, G.]]
[[Category: CA]]
[[Category: atp-grasp fold related]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:17:15 2007''

Latest revision as of 09:02, 21 February 2024

Crystal structure of Inositol Phosphate Multikinase Ipk2 from S. cerevisiae

2iew, resolution 2.00Å

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