2w8x: Difference between revisions
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==Structure of the tick ion-channel modulator Ra-KLP== | |||
<StructureSection load='2w8x' size='340' side='right'caption='[[2w8x]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2w8x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhipicephalus_appendiculatus Rhipicephalus appendiculatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W8X FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w8x OCA], [https://pdbe.org/2w8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w8x RCSB], [https://www.ebi.ac.uk/pdbsum/2w8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w8x ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ra-KLP, a 75 amino acid protein secreted by the salivary gland of the brown ear tick Rhipicephalus appendiculatus has a sequence resembling those of Kunitz/BPTI proteins. We report the detection, purification and characterization of the function of Ra-KLP. In addition, determination of the three-dimensional crystal structure of Ra-KLP at 1.6 A resolution using sulphur single-wavelength anomalous dispersion reveals that much of the loop structure of classical Kunitz domains, including the protruding protease-binding loop, has been replaced by beta-strands. Even more unusually, the N-terminal portion of the polypeptide chain is pinned to the "Kunitz head" by two disulphide bridges not found in classical Kunitz/BPTI proteins. The disulphide bond pattern has been further altered by the loss of the bridge that normally stabilizes the protease-binding loop. Consistent with the conversion of this loop into a beta-strand, Ra-KLP shows no significant anti-protease activity; however, it activates maxiK channels in an in vitro system, suggesting a potential mechanism for regulating host blood supply during feeding. | |||
An ion-channel modulator from the saliva of the brown ear tick has a highly modified Kunitz/BPTI structure.,Paesen GC, Siebold C, Dallas ML, Peers C, Harlos K, Nuttall PA, Nunn MA, Stuart DI, Esnouf RM J Mol Biol. 2009 Jun 19;389(4):734-47. Epub 2009 Apr 24. PMID:19394347<ref>PMID:19394347</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2w8x" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Rhipicephalus appendiculatus]] | |||
[[Category: Dallas M]] | |||
[[Category: Esnouf RM]] | |||
[[Category: Harlos K]] | |||
[[Category: Nunn MA]] | |||
[[Category: Nuttall PA]] | |||
[[Category: Paesen GC]] | |||
[[Category: Peers C]] | |||
[[Category: Siebold C]] | |||
[[Category: Stuart DI]] | |||
Latest revision as of 09:34, 6 November 2024
Structure of the tick ion-channel modulator Ra-KLP
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