2zrq: Difference between revisions

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{{Seed}}
[[Image:2zrq.jpg|left|200px]]


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==Crystal structure of S324A-subtilisin==
The line below this paragraph, containing "STRUCTURE_2zrq", creates the "Structure Box" on the page.
<StructureSection load='2zrq' size='340' side='right'caption='[[2zrq]], [[Resolution|resolution]] 2.16&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2zrq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_kodakarensis_KOD1 Thermococcus kodakarensis KOD1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZRQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZRQ FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
{{STRUCTURE_2zrq|  PDB=2zrq  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zrq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zrq OCA], [https://pdbe.org/2zrq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zrq RCSB], [https://www.ebi.ac.uk/pdbsum/2zrq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zrq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TKSU_THEKO TKSU_THEKO] Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zr/2zrq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zrq ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Tk-subtilisin (a subtilisin homologue from Thermococcus kodakaraensis) is matured from Pro-Tk-subtilisin upon autoprocessing and degradation of Tk-propeptide. To analyze the folding mechanism of Tk-subtilisin, the crystal structure of the active site mutant of Tk-subtilisin (S324A-subtilisin*), which was refolded in the presence of Ca2+ and absence of Tk-propeptide, was determined at 2.16A resolution. This structure is essentially the same as that of Tk-subtilisin matured from Pro-Tk-subtilisin. S324A-subtilisin* was refolded with a rate constant of 0.17 and 1.8min(-1) at 30 degrees C in the absence and presence of Tk-propeptide, respectively, indicating that Tk-subtilisin does not require Tk-propeptide for folding but requires it for acceleration of folding.


===Crystal structure of S324A-subtilisin===
Crystal structure of Tk-subtilisin folded without propeptide: requirement of propeptide for acceleration of folding.,Tanaka S, Takeuchi Y, Matsumura H, Koga Y, Takano K, Kanaya S FEBS Lett. 2008 Nov 26;582(28):3875-8. Epub 2008 Oct 23. PMID:18951896<ref>PMID:18951896</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2zrq" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_18951896}}, adds the Publication Abstract to the page
*[[Subtilisin 3D structures|Subtilisin 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 18951896 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_18951896}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Large Structures]]
2ZRQ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Thermococcus_kodakarensis Thermococcus kodakarensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZRQ OCA].
[[Category: Thermococcus kodakarensis KOD1]]
 
[[Category: Kanaya S]]
==Reference==
[[Category: Koga Y]]
<ref group="xtra">PMID:18951896</ref><references group="xtra"/>
[[Category: Matsumura H]]
[[Category: Subtilisin]]
[[Category: Takano K]]
[[Category: Thermococcus kodakarensis]]
[[Category: Takeuchi Y]]
[[Category: Kanaya, S.]]
[[Category: Tanaka S]]
[[Category: Koga, Y.]]
[[Category: Matsumura, H.]]
[[Category: Takano, K.]]
[[Category: Takeuchi, Y.]]
[[Category: Tanaka, S.]]
[[Category: Calcium]]
[[Category: Crystal structure]]
[[Category: Hydrolase]]
[[Category: Protease]]
[[Category: Secreted]]
[[Category: Serine protease]]
[[Category: Subtilisin]]
[[Category: Thermococcus kodakaraensis]]
[[Category: Zymogen]]
 
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Retrieved from "https://proteopedia.org/2zrq"