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New page: left|200px<br /><applet load="2ltn" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ltn, resolution 1.7Å" /> '''DESIGN, EXPRESSION, A...
 
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[[Image:2ltn.jpg|left|200px]]<br /><applet load="2ltn" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2ltn, resolution 1.7&Aring;" />
'''DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)'''<br />


==Overview==
==DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)==
The propeptide form of the lectin from the garden pea (Pisum sativum, agglutinin) has been expressed in Escherichia coli by attaching its cDNA, to an inducible promoter. By a number of criteria, including the ability, to form dimers, hemagglutination titer, Western blot, and enzyme-linked, immunosorbent assay, the resulting propeptide molecule is virtually, indistinguishable from the mature proteolytically processed lectin, isolated from peas. Preliminary crystallization experiments using the, recombinant propeptide lectin yield crystals in space group P2(1)2(1)2(1), with a = 64.8 A, b = 73.8 A, and c = 109.0 A (1 A = 0.1 nm) that diffract, to 2.8-A resolution. This unit cell size is quite similar to the unit cell, determined for native pea lectin, suggesting that the overall structure of, the recombinant prolectin is virtually identical.
<StructureSection load='2ltn' size='340' side='right'caption='[[2ltn]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2ltn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LTN FirstGlance]. <br>
2LTN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum] with MN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2LTN OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ltn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ltn OCA], [https://pdbe.org/2ltn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ltn RCSB], [https://www.ebi.ac.uk/pdbsum/2ltn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ltn ProSAT]</span></td></tr>
Design, expression, and crystallization of recombinant lectin from the garden pea (Pisum sativum)., Prasthofer T, Phillips SR, Suddath FL, Engler JA, J Biol Chem. 1989 Apr 25;264(12):6793-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2708344 2708344]
</table>
== Function ==
[https://www.uniprot.org/uniprot/LEC_PEA LEC_PEA] D-mannose specific lectin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lt/2ltn_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ltn ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pisum sativum]]
[[Category: Pisum sativum]]
[[Category: Protein complex]]
[[Category: Einspahr H]]
[[Category: Einspahr, H.]]
[[Category: Phillips SR]]
[[Category: Phillips, S.R.]]
[[Category: Suddath FL]]
[[Category: Suddath, F.L.]]
[[Category: CA]]
[[Category: MN]]
[[Category: lectin]]
 
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