2kdc: Difference between revisions

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'''Unreleased structure'''


The entry 2kdc is ON HOLD  until Paper Publication
==NMR Solution Structure of E. coli diacylglycerol kinase (DAGK) in DPC micelles==
<StructureSection load='2kdc' size='340' side='right'caption='[[2kdc]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2kdc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KDC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KDC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kdc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kdc OCA], [https://pdbe.org/2kdc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kdc RCSB], [https://www.ebi.ac.uk/pdbsum/2kdc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kdc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KDGL_ECOLI KDGL_ECOLI] Recycling of diacylglycerol produced during the turnover of membrane phospholipid.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kd/2kdc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kdc ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Escherichia coli diacylglycerol kinase (DAGK) represents a family of integral membrane enzymes that is unrelated to all other phosphotransferases. We have determined the three-dimensional structure of the DAGK homotrimer with the use of solution nuclear magnetic resonance. The third transmembrane helix from each subunit is domain-swapped with the first and second transmembrane segments from an adjacent subunit. Each of DAGK's three active sites resembles a portico. The cornice of the portico appears to be the determinant of DAGK's lipid substrate specificity and overhangs the site of phosphoryl transfer near the water-membrane interface. Mutations to cysteine that caused severe misfolding were located in or near the active site, indicating a high degree of overlap between sites responsible for folding and for catalysis.


Authors: Van Horn, W.D., Kim, H., Ellis, C.D., Hadziselimovic, A., Sulistijo, E.S., Karra, M.D., Tian, C., Sonnichsen, F.D., Sanders, C.R.
Solution nuclear magnetic resonance structure of membrane-integral diacylglycerol kinase.,Van Horn WD, Kim HJ, Ellis CD, Hadziselimovic A, Sulistijo ES, Karra MD, Tian C, Sonnichsen FD, Sanders CR Science. 2009 Jun 26;324(5935):1726-9. PMID:19556511<ref>PMID:19556511</ref>


Description: NMR Solution Structure of E. coli diacylglycerol kinase (DAGK) in DPC micelles
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2kdc" style="background-color:#fffaf0;"></div>


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 22 10:21:44 2009''
==See Also==
*[[Diacylglycerol kinase 3D structures|Diacylglycerol kinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Ellis CD]]
[[Category: Hadziselimovic A]]
[[Category: Karra MD]]
[[Category: Kim H]]
[[Category: Sanders CR]]
[[Category: Sonnichsen FD]]
[[Category: Sulistijo ES]]
[[Category: Tian C]]
[[Category: Van Horn WD]]
Retrieved from "https://proteopedia.org/2kdc"