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New page: left|200px<br /> <applet load="1ckm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ckm, resolution 2.5Å" /> '''STRUCTURE OF TWO DIF...
 
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[[Image:1ckm.gif|left|200px]]<br />
<applet load="1ckm" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ckm, resolution 2.5&Aring;" />
'''STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP'''<br />


==Overview==
==STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP==
We have solved the crystal structure of an mRNA capping enzyme at 2.5 A, resolution. The enzyme comprises two domains with a deep, but narrow, cleft between them. The two molecules in the crystallographic asymmetric, unit adopt very different conformations; both contain a bound GTP, but one, protein molecule is in an open conformation while the other is in a closed, conformation. Only in the closed conformation is the enzyme able to bind, manganese ions and undergo catalysis within the crystals to yield the, covalent guanylated enzyme intermediate. These structures provide direct, evidence for a mechanism that involves a significant conformational change, in the enzyme during catalysis.
<StructureSection load='1ckm' size='340' side='right'caption='[[1ckm]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1ckm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_1 Paramecium bursaria Chlorella virus 1]. The January 2012 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Messenger RNA Capping''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2012_1 10.2210/rcsb_pdb/mom_2012_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CKM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CKM FirstGlance]. <br>
1CKM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]] with GTP as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.50 2.7.7.50]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CKM OCA]].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ckm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ckm OCA], [https://pdbe.org/1ckm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ckm RCSB], [https://www.ebi.ac.uk/pdbsum/1ckm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ckm ProSAT]</span></td></tr>
X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes., Hakansson K, Doherty AJ, Shuman S, Wigley DB, Cell. 1997 May 16;89(4):545-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9160746 9160746]
</table>
[[Category: Paramecium bursaria chlorella virus 1]]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/MCE_PBCV1 MCE_PBCV1] mRNA capping. Transfers a GMP cap onto the end of mRNA that terminates with a 5'-diphosphate tail.
[[Category: Doherty, A.J.]]
== Evolutionary Conservation ==
[[Category: Hakansson, K.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Wigley, D.B.]]
Check<jmol>
[[Category: GTP]]
  <jmolCheckbox>
[[Category: capping enzyme]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ck/1ckm_consurf.spt"</scriptWhenChecked>
[[Category: mrna]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: nucleotidyltransferase]]
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 22:14:10 2007''
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ckm ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Messenger RNA Capping]]
[[Category: Paramecium bursaria Chlorella virus 1]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Doherty AJ]]
[[Category: Hakansson K]]
[[Category: Wigley DB]]

Latest revision as of 06:43, 7 February 2024

STRUCTURE OF TWO DIFFERENT CONFORMATIONS OF MRNA CAPPING ENZYME IN COMPLEX WITH GTP

1ckm, resolution 2.50Å

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