3fh6: Difference between revisions

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{{Seed}}
[[Image:3fh6.png|left|200px]]


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==Crystal structure of the resting state maltose transporter from E. coli==
The line below this paragraph, containing "STRUCTURE_3fh6", creates the "Structure Box" on the page.
<StructureSection load='3fh6' size='340' side='right'caption='[[3fh6]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3fh6]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FH6 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fh6 OCA], [https://pdbe.org/3fh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fh6 RCSB], [https://www.ebi.ac.uk/pdbsum/3fh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fh6 ProSAT]</span></td></tr>
{{STRUCTURE_3fh6|  PDB=3fh6  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/MALF_ECOLI MALF_ECOLI] Part of the binding-protein-dependent transport system for maltose; probably responsible for the translocation of the substrate across the membrane.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fh/3fh6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fh6 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
ATP-binding cassette transporters couple ATP hydrolysis to substrate translocation through an alternating access mechanism, but the nature of the conformational changes in a transport cycle remains elusive. Previously we reported the structure of the maltose transporter MalFGK(2) in an outward-facing conformation in which the transmembrane (TM) helices outline a substrate-binding pocket open toward the periplasmic surface and ATP is poised for hydrolysis along the closed nucleotide-binding dimer interface. Here we report the structure of the nucleotide-free maltose transporter in which the substrate binding pocket is only accessible from the cytoplasm and the nucleotide-binding interface is open. Comparison of the same transporter crystallized in two different conformations reveals that alternating access involves rigid-body rotations of the TM subdomains that are coupled to the closure and opening of the nucleotide-binding domain interface. The comparison also reveals that point mutations enabling binding protein-independent transport line dynamic interfaces in the TM region.


===Crystal structure of the resting state maltose transporter from E. coli===
Alternating access in maltose transporter mediated by rigid-body rotations.,Khare D, Oldham ML, Orelle C, Davidson AL, Chen J Mol Cell. 2009 Feb 27;33(4):528-36. PMID:19250913<ref>PMID:19250913</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3fh6" style="background-color:#fffaf0;"></div>


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==See Also==
The line below this paragraph, {{ABSTRACT_PUBMED_19250913}}, adds the Publication Abstract to the page
*[[Maltose-binding protein 3D structures|Maltose-binding protein 3D structures]]
(as it appears on PubMed at http://www.pubmed.gov), where 19250913 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_19250913}}
__TOC__
 
</StructureSection>
==About this Structure==
[[Category: Escherichia coli K-12]]
3FH6 is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FH6 OCA].
[[Category: Large Structures]]
 
[[Category: Chen J]]
==Reference==
[[Category: Davidson AL]]
<ref group="xtra">PMID:19250913</ref><references group="xtra"/>
[[Category: Khare D]]
[[Category: Escherichia coli k-12]]
[[Category: Oldham ML]]
[[Category: Maltose-transporting ATPase]]
[[Category: Orelle C]]
[[Category: Chen, J.]]
[[Category: Davidson, A L.]]
[[Category: Khare, D.]]
[[Category: Oldham, M L.]]
[[Category: Orelle, C.]]
[[Category: Abc transporter]]
[[Category: Atp-binding]]
[[Category: Cell inner membrane]]
[[Category: Cell membrane]]
[[Category: Ground state]]
[[Category: Hydrolase]]
[[Category: Maltose transporter]]
[[Category: Membrane]]
[[Category: Membrane protein]]
[[Category: Nucleotide-binding]]
[[Category: Sugar transport]]
[[Category: Transmembrane]]
[[Category: Transport]]
[[Category: Transport protein]]
 
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Retrieved from "https://proteopedia.org/3fh6"